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4 hits found for Toyama

SASDKL4 – High temperature requirement of human mitochondrial serine protease HTRA2 (S306A mutant; 10 mg/ml)

Serine protease HTRA2, mitochondrial experimental SAS data
SASREF MX model
Sample: Serine protease HTRA2, mitochondrial hexamer, 210 kDa Homo sapiens protein
Buffer: 20 mM HEPES-NaOH (pH 7.4), 120 mM NaCl, 1 mM EDTA, and 2% glycerol, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Jul 1
Oligomeric assembly regulating mitochondrial HtrA2 function as examined by methyl-TROSY NMR Proceedings of the National Academy of Sciences 118(11):e2025022118 (2021)
Toyama Y, Harkness R, Lee T, Maynes J, Kay L
RgGuinier 4.2 nm
Dmax 16.0 nm
VolumePorod 250 nm3

SASDKM4 – High temperature requirement of human mitochondrial serine protease HTRA2 (S306A mutant; 5 mg/ml)

Serine protease HTRA2, mitochondrial experimental SAS data
SASREF MX model
Sample: Serine protease HTRA2, mitochondrial hexamer, 210 kDa Homo sapiens protein
Buffer: 20 mM HEPES-NaOH (pH 7.4), 120 mM NaCl, 1 mM EDTA, and 2% glycerol, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Jul 1
Oligomeric assembly regulating mitochondrial HtrA2 function as examined by methyl-TROSY NMR Proceedings of the National Academy of Sciences 118(11):e2025022118 (2021)
Toyama Y, Harkness R, Lee T, Maynes J, Kay L
RgGuinier 3.8 nm
Dmax 15.0 nm
VolumePorod 213 nm3

SASDKN4 – High temperature requirement of human mitochondrial serine protease HTRA2 (S306A mutant; 2 mg/ml)

Serine protease HTRA2, mitochondrial experimental SAS data
SASREF MX model
Sample: Serine protease HTRA2, mitochondrial hexamer, 210 kDa Homo sapiens protein
Buffer: 20 mM HEPES-NaOH (pH 7.4), 120 mM NaCl, 1 mM EDTA, and 2% glycerol, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Jul 1
Oligomeric assembly regulating mitochondrial HtrA2 function as examined by methyl-TROSY NMR Proceedings of the National Academy of Sciences 118(11):e2025022118 (2021)
Toyama Y, Harkness R, Lee T, Maynes J, Kay L
RgGuinier 3.6 nm
Dmax 12.8 nm
VolumePorod 203 nm3

SASDKP4 – High temperature requirement of human mitochondrial serine protease HTRA2 (S306A mutant; 1 mg/ml)

Serine protease HTRA2, mitochondrial experimental SAS data
SASREF MX model
Sample: Serine protease HTRA2, mitochondrial hexamer, 210 kDa Homo sapiens protein
Buffer: 20 mM HEPES-NaOH (pH 7.4), 120 mM NaCl, 1 mM EDTA, and 2% glycerol, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Jul 1
Oligomeric assembly regulating mitochondrial HtrA2 function as examined by methyl-TROSY NMR Proceedings of the National Academy of Sciences 118(11):e2025022118 (2021)
Toyama Y, Harkness R, Lee T, Maynes J, Kay L
RgGuinier 3.4 nm
Dmax 12.0 nm
VolumePorod 168 nm3