Acetyl-CoA decarbonylase/synthase (ACDS) from Methanosarcina acetivorans

Erik Zimmer.

SASD2C2 – Acetyl-CoA decarbonylase/synthase (ACDS), natively purified from Methanosarcina acetivorans

Acetyl-CoA decarbonylase/synthase complex subunit alpha 2
Acetyl-CoA decarbonylase/synthase complex subunit epsilon 2
Acetyl-CoA decarbonylase/synthase complex subunit beta 2
Acetyl-CoA decarbonylase/synthase complex subunit delta 2
Acetyl-CoA decarbonylase/synthase complex subunit gamma
MWexperimental 2182 kDa
MWexpected 2054 kDa
log I(s) 5.63×102 5.63×101 5.63×100 5.63×10-1
Acetyl-CoA decarbonylase/synthase complex subunit alpha 2 Acetyl-CoA decarbonylase/synthase complex subunit epsilon 2 Acetyl-CoA decarbonylase/synthase complex subunit beta 2 Acetyl-CoA decarbonylase/synthase complex subunit delta 2 Acetyl-CoA decarbonylase/synthase complex subunit gamma small angle scattering data  s, nm-1
ln I(s)
Acetyl-CoA decarbonylase/synthase complex subunit alpha 2 Acetyl-CoA decarbonylase/synthase complex subunit epsilon 2 Acetyl-CoA decarbonylase/synthase complex subunit beta 2 Acetyl-CoA decarbonylase/synthase complex subunit delta 2 Acetyl-CoA decarbonylase/synthase complex subunit gamma Guinier plot ln 5.63×102 Rg: 10.9 nm 0 (10.9 nm)-2 s2
(sRg)2I(s)/I(0)
Acetyl-CoA decarbonylase/synthase complex subunit alpha 2 Acetyl-CoA decarbonylase/synthase complex subunit epsilon 2 Acetyl-CoA decarbonylase/synthase complex subunit beta 2 Acetyl-CoA decarbonylase/synthase complex subunit delta 2 Acetyl-CoA decarbonylase/synthase complex subunit gamma Kratky plot 1.104 0 √3 sRg
p(r)
Acetyl-CoA decarbonylase/synthase complex subunit alpha 2 Acetyl-CoA decarbonylase/synthase complex subunit epsilon 2 Acetyl-CoA decarbonylase/synthase complex subunit beta 2 Acetyl-CoA decarbonylase/synthase complex subunit delta 2 Acetyl-CoA decarbonylase/synthase complex subunit gamma pair distance distribution function Rg: 10.6 nm 0 Dmax: 31.4 nm

Data validation

There are no models related to this curve.

Each of the Acetyl-CoA decarbonylase/synthase five subunits (alpha, beta, gamma, delta, epsilon) occurs eight times in the complex, so the total stoichiometry is CdhA8B8C8D8E8.

Acetyl-CoA decarbonylase/synthase complex subunit alpha 2 (CdhA)
Mol. type   Protein
Organism   Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Olig. state   Other
Mon. MW   88.0 kDa
 
UniProt   Q8TJC6
Sequence   FASTA
 
Acetyl-CoA decarbonylase/synthase complex subunit epsilon 2 (CdhB)
Mol. type   Protein
Organism   Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Olig. state   Other
Mon. MW   18.5 kDa
 
UniProt   Q8TJC5
Sequence   FASTA
 
Acetyl-CoA decarbonylase/synthase complex subunit beta 2 (CdhC)
Mol. type   Protein
Organism   Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Olig. state   Other
Mon. MW   52.4 kDa
 
UniProt   Q8TJC4
Sequence   FASTA
 
Acetyl-CoA decarbonylase/synthase complex subunit delta 2 (CdhD)
Mol. type   Protein
Organism   Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Olig. state   Other
Mon. MW   47.1 kDa
 
UniProt   Q8TJC2
Sequence   FASTA
 
Acetyl-CoA decarbonylase/synthase complex subunit gamma (CdhE)
Mol. type   Protein
Organism   Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Olig. state   Other
Mon. MW   50.8 kDa
 
UniProt   Q8TH44
Sequence   FASTA