Global Lrp regulator protein from Haloferax mediterranei: Transcriptional analysis and structural characterization.

Matarredona L, García-Bonete MJ, Guío J, Camacho M, Fillat MF, Esclapez J, Bonete MJ, Int J Biol Macromol :129541 (2024) Europe PMC

SASDT82 – Halophilic Lrp transcription factor

AsnC family transcriptional regulator

MW^experimental	32	kDa
MW^expected	36	kDa
V^Porod	70	nm³

log I(s) 2.58×10¹ 2.58×10⁰ 2.58×10^-1 2.58×10^-2

AsnC family transcriptional regulator small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

AsnC family transcriptional regulator Guinier plot

ln 2.58×10¹ R_g: 2.4 nm 0 (2.4 nm)^-2 s²

(sR_g)²I(s)/I(0)

AsnC family transcriptional regulator Kratky plot

1.104 0 √3 sR_g

p(r)	R_g: 2.4 nm 0 D_max: 7.9 nm

Data validation

Experimental data range

p(r) fit to data

Metric

Value

s_min D_max / π

0.2

N_Shannon

Worse

Better

Metric

Value

p_cormap

χ²

0.457
1.071

Worse

Better

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

AsnC family transcriptional regulator PHENIX model

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

AsnC family transcriptional regulator DAMMIN model

Synchrotron SAXS data from solutions of halophilic Lrp transcription factor in 50 mM Tris, 1.5 M NaCl, pH 8 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 20°C. One 1 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

AsnC family transcriptional regulator (Lrp)
Mol. type		Protein
Organism		Haloferax mediterranei (strain ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4)
Olig. state		Dimer
Mon. MW		17.8 kDa

UniProt		I3R177 (1-152)
Sequence		FASTA