Self-assembly and regulation of protein cages from pre-organised coiled-coil modules.

Lapenta F, Aupič J, Vezzoli M, Strmšek Ž, Da Vela S, Svergun DI, Carazo JM, Melero R, Jerala R, Nat Commun 12(1):939 (2021) Europe PMC

SASDJU5 – BIP18SN

BIP18SN
MWI(0) 94 kDa
MWexpected 80 kDa
log I(s) 6.12×103 6.12×102 6.12×101 6.12×100
BIP18SN small angle scattering data  s, nm-1
ln I(s)
BIP18SN Guinier plot ln 6.13×103 Rg: 4.6 nm 0 (4.6 nm)-2 s2
(sRg)2I(s)/I(0)
BIP18SN Kratky plot 1.104 0 √3 sRg
p(r)
BIP18SN pair distance distribution function Rg: 4.2 nm 0 Dmax: 12.4 nm

Data validation

Fits and models

log I(s)
 s, nm-1
BIP18SN MODELLER model
Synchrotron SAXS data from solutions of BIP18SN in 20 mM Tris 150 mM NaCl 10% glycerol, pH 7.5 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1 and 10 mg/ml were measured at 20°C. 40 successive 0.045 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

BIP18SN
Mol. type   Protein
Organism   synthetic construct
Olig. state   Monomer
Mon. MW   79.8 kDa
Sequence   FASTA