Human cathepsin X/Z is a biologically active homodimer.

Dolenc I, Štefe I, Turk D, Taler-Verčič A, Turk B, Turk V, Stoka V, Biochim Biophys Acta Proteins Proteom :140567 (2020) Europe PMC

SASDJQ7 – Human Cathepsin X

Cathepsin Z
MWexperimental 53 kDa
MWexpected 54 kDa
VPorod 89 nm3
log I(s) 9.45×103 9.45×102 9.45×101 9.45×100
Cathepsin Z small angle scattering data  s, nm-1
ln I(s)
Cathepsin Z Guinier plot ln 9.45×103 Rg: 2.6 nm 0 (2.6 nm)-2 s2
(sRg)2I(s)/I(0)
Cathepsin Z Kratky plot 1.104 0 √3 sRg
p(r)
Cathepsin Z pair distance distribution function Rg: 2.4 nm 0 Dmax: 6.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Cathepsin Z PDB (PROTEIN DATA BANK) model
Synchrotron SAXS data from solutions of Human Cathepsin X in 20 mM sodium acetate, 1 mM EDTA, pH 5.5 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.5 and 1.5 mg/ml were measured at 20°C. 40 successive 0.045 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Cathepsin Z (CTSZ)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   27.1 kDa
 
UniProt   Q9UBR2 (62-303)
Sequence   FASTA
 
PDB ID   1EF7