| MWI(0) | 72 | kDa |
| MWexpected | 85 | kDa |
| VPorod | 125 | nm3 |
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log I(s)
5.56×10-2
5.56×10-3
5.56×10-4
5.56×10-5
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s, nm-1
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The missing part: the Archaeoglobus fulgidus Argonaute forms a functional heterodimer with an N-L1-L2 domain protein.
Manakova E Golovinas E, Pocevičiūtė R, Sasnauskas G, Silanskas A, Rutkauskas D, Jankunec M, Zagorskaitė E, Jurgelaitis E, Grybauskas A, Venclovas Č, Zaremba M, Nucleic Acids Res (2024) Europe PMCSASDRZ8 – Single-chain Archaeoglobus fulgidus argonaute fAfAgo-fusion variant complexed with an 11 base pair DNA oligoduplex in low salt buffer
single-chain full Archaeoglobus fulgidus Argonaute5'-end phosphorylated DNA guide strand, 11 nt (MZ864)
DNA target strand, 11 nt (MZ865)
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Fits and models
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Synchrotron SAXS data from solutions of a fAfAgo fusion protein complexed with an 11 base pair DNA oligoduplex in 20 mM TrisHCl pH7.5, 200 mM NaCl, 5 mM MgCl2, 1 mM DTT, were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.80 mg/ml was measured at 20°C. 20 successive 0.045 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
DNA oligoduplex was mixed with scfAfAgo in the presence of MgCl2, buffer was changed using NAP Illustra column and concentrated to 3.8 mg/ml. Note: The DNA sequence of the model shown in this entry is different to the DNA sequence of the oligoduplex in the sample used for SAXS. |
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