Dmax unknown – experimental data range validation not possible.
There are no models related to this curve.
Synchrotron SAXS
data from solutions of
Full-length yeast shuttle protein Dsk2 with HS2 deletion (residues 279-291)
in
20 mM sodium phosphate, 0.5 mM EDTA, 0.02% NaN3, pH 6.8
were collected
on the
BioCAT 18ID beam line
at the Advanced Photon Source (APS), Argonne National Laboratory storage ring
(Lemont, IL, USA)
using a Pilatus3 X 1M detector
at a sample-detector distance of 3.7 m and
at a wavelength of λ = 1.0332 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 200.00 μl sample
at 6.3 mg/ml was injected at a 0.60 ml/min flow rate
onto a GE Superdex 200 Increase 10/300 column
at 23.7°C.
2470 successive
0.500 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
s, nm-1
