Complex of Rat1-Rai1-Rtt103 from Yeast

Tomas Klumpler.

SASDU49 – Complex of Ribonucleic Acid Trafficking (Rat1) and Rat1 Interacting Protein 1 (Rai1) and Regulator of Ty1 transposition protein 103 (Rtt103) from Yeast

Regulator of Ty1 transposition protein 103
Ribonucleic Acid Trafficking 5'-3' exoribonuclease 2
Decapping nuclease RAI1
MWexperimental 318 kDa
MWexpected 378 kDa
VPorod 1010 nm3
log I(s) 3.64×10-1 3.64×10-2 3.64×10-3 3.64×10-4
Regulator of Ty1 transposition protein 103 Ribonucleic Acid Trafficking 5'-3' exoribonuclease 2 Decapping nuclease RAI1 small angle scattering data  s, nm-1
ln I(s)
Regulator of Ty1 transposition protein 103 Ribonucleic Acid Trafficking 5'-3' exoribonuclease 2 Decapping nuclease RAI1 Guinier plot ln 3.65×10-1 Rg: 8.7 nm 0 (8.7 nm)-2 s2
(sRg)2I(s)/I(0)
Regulator of Ty1 transposition protein 103 Ribonucleic Acid Trafficking 5'-3' exoribonuclease 2 Decapping nuclease RAI1 Kratky plot 1.104 0 √3 sRg
p(r)
Regulator of Ty1 transposition protein 103 Ribonucleic Acid Trafficking 5'-3' exoribonuclease 2 Decapping nuclease RAI1 pair distance distribution function Rg: 9.0 nm 0 Dmax: 26.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Regulator of Ty1 transposition protein 103 Ribonucleic Acid Trafficking 5'-3' exoribonuclease 2 Decapping nuclease RAI1 CORAL model
SAXS data from solutions of the Rat1/Rai1/Rtt103 complex in 25 mM Tris, 120 mM NaCl, 5% glycerol, 2 mM MgSO4, pH 8 were collected using a Rigaku BioSAXS-2000 instrument at CEITEC (Brno, Czech Republic) equipped with Rigaku HyPix-3000 detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 4°C. Nine successive 600 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Regulator of Ty1 transposition protein 103
Mol. type   Protein
Organism   Saccharomyces cerevisiae
Olig. state   Dimer
Mon. MW   28.6 kDa
 
UniProt   Q05543 (1-246)
Sequence   FASTA
 
Ribonucleic Acid Trafficking 5'-3' exoribonuclease 2 (Rat1)
Mol. type   Protein
Organism   Saccharomyces cerevisiae
Olig. state   Other
Mon. MW   115.9 kDa
 
UniProt   Q02792 (1-1006)
Sequence   FASTA
 
Decapping nuclease RAI1 (Rai1)
Mol. type   Protein
Organism   Saccharomyces cerevisiae
Olig. state   Other
Mon. MW   44.5 kDa
 
UniProt   P53063 (1-387)
Sequence   FASTA