| MWexperimental | 32 | kDa |
| MWexpected | 33 | kDa |
|
log I(s)
3.02×101
3.02×100
3.02×10-1
3.02×10-2
|
s, nm-1
|
Use of biomolecular emulator for characterizing flexible proteins by small-angle x-ray scattering.
Haitin Y, Manori B, Giladi M Protein Sci 35(6):e70646 (2026) Europe PMCSASDZD3 – Double C2-like domain-containing protein beta (DOC2B) C2AB domains from , apo state
Double C2-like domain-containing protein beta|
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Fits and models
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Rg, nm
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Synchrotron SAXS
data from solutions of
Double C2-like domain-containing protein beta (DOC2B) C2AB domains from , apo state
in
20 mM Tris–HCl, 200 mM NaCl, 20 mM β-mercaptoethanol, 10 mM EDTA, pH 6.8
were collected
on the
EMBL X33 beam line
at the DORIS III, DESY storage ring
(Hamburg, Germany)
using a Pilatus 1M-W detector
at a sample-detector distance of 2.7 m and
at a wavelength of λ = 0.15 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 2 and 10 mg/ml were measured
at 5°C.
10 successive
2 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
Tags:
X33
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