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3 hits found for Histone H3 variant

SASDX83 – T. brucei Canonical Nucleosome Core Particle

Histone H2AHistone H2BHistone H3, putativeHistone H4Widom 601 145 bp DNA - strand 1Widom 601 145 bp DNA - strand 2 experimental SAS data
Histone H2A Histone H2B Histone H3, putative Histone H4 Widom 601 145 bp DNA - strand 1 Widom 601 145 bp DNA - strand 2 Kratky plot
Sample: Histone H2A , 28 kDa Trypanosoma brucei brucei … protein
Histone H2B , 25 kDa Trypanosoma brucei brucei … protein
Histone H3, putative , 29 kDa Trypanosoma brucei brucei … protein
Histone H4 , 22 kDa Trypanosoma brucei brucei … protein
Widom 601 145 bp DNA - strand 1 , 45 kDa DNA
Widom 601 145 bp DNA - strand 2 , 45 kDa DNA
Buffer: 20 mM HEPES, 150 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Oct 10
...histone variants H3.V and H4.V promote nucleosome plasticity in repressed chromatin
RgGuinier 4.3 nm
Dmax 11.8 nm
VolumePorod 435 nm3

SASDX93 – ...H3.V+H4.V Nucleosome Core Particle

Histone H2AHistone H2BWidom 601 145 bp DNA - strand 1Widom 601 145 bp DNA - strand 2Histone H3 variantHistone H4 experimental SAS data
Histone H2A Histone H2B Widom 601 145 bp DNA - strand 1 Widom 601 145 bp DNA - strand 2 Histone H3 variant Histone H4 Kratky plot
Sample: Histone H2A , 28 kDa Trypanosoma brucei brucei … protein
Histone H2B , 25 kDa Trypanosoma brucei brucei … protein
Widom 601 145 bp DNA - strand 1 , 45 kDa DNA
Widom 601 145 bp DNA - strand 2 , 45 kDa DNA
Histone H3 variant , 32 kDa Trypanosoma brucei brucei … protein
Histone H4 , 22 kDa Trypanosoma brucei brucei … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Oct 10
...histone variants H3.V and H4.V promote nucleosome plasticity in repressed chromatin
RgGuinier 4.5 nm
Dmax 13.1 nm
VolumePorod 483 nm3

SASDXA3 – ...H3.V-only Nucleosome Core Particle

Histone H2AHistone H2BHistone H4Widom 601 145 bp DNA - strand 1Widom 601 145 bp DNA - strand 2Histone H3 variant experimental SAS data
Histone H2A Histone H2B Histone H4 Widom 601 145 bp DNA - strand 1 Widom 601 145 bp DNA - strand 2 Histone H3 variant Kratky plot
Sample: Histone H2A , 28 kDa Trypanosoma brucei brucei … protein
Histone H2B , 25 kDa Trypanosoma brucei brucei … protein
Histone H4 , 22 kDa Trypanosoma brucei brucei … protein
Widom 601 145 bp DNA - strand 1 , 45 kDa DNA
Widom 601 145 bp DNA - strand 2 , 45 kDa DNA
Histone H3 variant , 32 kDa Trypanosoma brucei brucei … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Oct 10
...histone variants H3.V and H4.V promote nucleosome plasticity in repressed chromatin
Gauri Deak
RgGuinier 4.4 nm
Dmax 13.2 nm
VolumePorod 481 nm3