An integrative approach unveils a distal encounter site for rPTPε and phospho-Src complex formation

EswarKumar N, Yang C, Tewary S, Peng W, Chen G, Yeh Y, Yang H, Ho M, Structure (2023) DOI

SASDJ33 – Receptor-type tyrosine-protein phosphatase epsilon bound to phospho proto-oncogene tyrosine-protein kinase (rPTPε/phospho-Src complex)

Receptor-type tyrosine-protein phosphatase epsilon
Proto-oncogene tyrosine-protein kinase Src, T357M mutant
MWexperimental 68 kDa
MWexpected 66 kDa
VPorod 60 nm3
log I(s) 4.40×10-2 4.40×10-3 4.40×10-4 4.40×10-5
Receptor-type tyrosine-protein phosphatase epsilon Proto-oncogene tyrosine-protein kinase Src, T357M mutant small angle scattering data  s, nm-1
ln I(s)
Receptor-type tyrosine-protein phosphatase epsilon Proto-oncogene tyrosine-protein kinase Src, T357M mutant Guinier plot ln 4.40×10-2 Rg: 2.9 nm 0 (2.9 nm)-2 s2
(sRg)2I(s)/I(0)
Receptor-type tyrosine-protein phosphatase epsilon Proto-oncogene tyrosine-protein kinase Src, T357M mutant Kratky plot 1.104 0 √3 sRg
p(r)
Receptor-type tyrosine-protein phosphatase epsilon Proto-oncogene tyrosine-protein kinase Src, T357M mutant pair distance distribution function Rg: 3.0 nm 0 Dmax: 10 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Receptor-type tyrosine-protein phosphatase epsilon Proto-oncogene tyrosine-protein kinase Src, T357M mutant CORAL model
Synchrotron SAXS data from solutions of the rPTPε/phospho-Src complex in 20 mM Tris , 50 mM NaCl, 5 mM DTT, pH 8 were collected on the 23A1 beam line at the NSRRC (Hshinchu, Taiwan) using a MAR 165 detector at a sample-detector distance of 2.6 m and at a wavelength of λ = 0.08267 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 6.00 mg/ml was measured at 15°C. Five successive 330 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

The displayed model is without the 6-His tag at the N-terminus of phospho-Src and few residues are missing in the N-terminus of rPTPε.

Receptor-type tyrosine-protein phosphatase epsilon (rPTPε)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   34.1 kDa
 
UniProt   P23469 (107-400)
Sequence   FASTA
 
Proto-oncogene tyrosine-protein kinase Src, T357M mutant (Src(T357M))
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   31.5 kDa
 
UniProt   P12931 (263-536)
Sequence   FASTA