| MWexperimental | 105 | kDa |
| MWexpected | 100 | kDa |
| VPorod | 154 | nm3 |
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log I(s)
4.90×10-2
4.90×10-3
4.90×10-4
4.90×10-5
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s, nm-1
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Multi-domain O-GlcNAcase structures reveal allosteric regulatory mechanisms.
Hansen SB, Bartual SG, Yuan H, Raimi OG, Gorelik A, Ferenbach AT, Lytje K, Pedersen JS, Drace T, Boesen T, van Aalten DMF, Nat Commun 16(1):8828 (2025) Europe PMCSASDXF3 – RTX cytotoxin pro-MbxA without acylation (pro-MbxA)
RTX cytotoxin pro-MbxA without acylation|
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Fits and models
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Synchrotron SAXS
data from solutions of
RTX cytotoxin pro-MbxA without acylation (pro-MbxA)
in
50 mM Tris, 100 mM NaCl, 10mM CaCl2, pH 7.8
were collected
on the
EMBL P12 beam line
at the PETRA III storage ring
(DESY; Hamburg, Germany)
using a Pilatus 6M detector
at a sample-detector distance of 3 m and
at a wavelength of λ = 0.155 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample
at 5.3 mg/ml was injected at a 0.50 ml/min flow rate
onto a GE Superose 6 Increase 10/300 column
at 20°C.
3000 successive
0.995 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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