| MWexperimental | 303 | kDa |
| MWexpected | 319 | kDa |
| VPorod | 457 | nm3 |
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log I(s)
2.69×101
2.69×100
2.69×10-1
2.69×10-2
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s, nm-1
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Combination of SAXS and Protein Painting Discloses the Three-Dimensional Organization of the Bacterial Cysteine Synthase Complex, a Potential Target for Enhancers of Antibiotic Action.
Rosa B, Marchetti M, Paredi G, Amenitsch H Franko N, Benoni R, Giabbai B, De Marino MG, Mozzarelli A, Ronda L, Storici P, Campanini B, Bettati S, Int J Mol Sci 20(20) (2019) Europe PMCSASDGX6 – Cysteine synthase/Serine acetyltransferase complex
Cysteine synthase A (4-mer)Serine acetyltransferase (6-mer)
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Fits and models
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Synchrotron SAXS data from solutions of the cysteine synthase complex in 20 mM sodium phosphate, 85 mM NaCl, 2 mM EDTA, 10 mM 2-MCE, pH 7.5 were collected on the Austrian SAXS beamline 5.2L beam line at the ELETTRA - Sincrotrone Trieste storage ring (Trieste, Italy) using a Dectris / Pilatus3 1M detector at a sample-detector distance of 1.2 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.5 and 1.5 mg/ml were measured at 20°C. 12 successive 10 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
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