Solution characterization of TrhW, a type 4 secretion system regulatory protein from the R27 plasmid

Rodriguez C Audette G, Structural Dynamics 13(5) (2026) DOI

SASDZA4 – Putative transfer and pilus formation protein, TrhW, from the R27 plasmid of Salmonella typhi.

TrhW
MWexperimental 60 kDa
MWexpected 57 kDa
VPorod 104 nm3
log I(s) 1.62×10-2 1.62×10-3 1.62×10-4 1.62×10-5
TrhW small angle scattering data  s, nm-1
ln I(s)
TrhW Guinier plot ln 1.63×10-2 Rg: 4.4 nm 0 (4.4 nm)-2 s2
(sRg)2I(s)/I(0)
TrhW Kratky plot 1.104 0 √3 sRg
p(r)
TrhW pair distance distribution function Rg: 4.5 nm 0 Dmax: 16 nm

Data validation

Fits and models

log I(s)
 s, nm-1
TrhW DAMMIF model
Synchrotron SAXS data from solutions of Putative transfer and pilus formation protein, TrhW, from the R27 plasmid of Salmonella typhi. in 10 mM HEPES, 100 mM NaCl, pH 7 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS), Argonne National Laboratory storage ring (Lemont, IL, USA) using a Eiger2 XE 9M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 230.00 μl sample at 5 mg/ml was injected at a 0.60 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Number of frames = UNKNOWN

TrhW
Mol. type   Protein
Organism   Salmonella enterica subsp. enterica serovar Typhi
Olig. state   Monomer
Mon. MW   57.3 kDa
 
UniProt   Q9RGV6 (1-502)
Sequence   FASTA