Unique structural and ligand-binding properties of the Staphylococcus aureus serine hydrolase FphE

Jo J, Upadhyay T, You X Bennett J, Lee H, Bogyo M, Fellner M, Proceedings of the National Academy of Sciences 123(13) (2026) DOI

SASDX64 – Carboxylic ester hydrolase FphE

Uncharacterized hydrolase SAUSA300_2518
MWexperimental 57 kDa
MWexpected 62 kDa
VPorod 84 nm3
log I(s) 2.91×10-3 2.91×10-4 2.91×10-5 2.91×10-6
Uncharacterized hydrolase SAUSA300_2518 small angle scattering data  s, nm-1
ln I(s)
Uncharacterized hydrolase SAUSA300_2518 Guinier plot ln 2.92×10-3 Rg: 3.0 nm 0 (3.0 nm)-2 s2
(sRg)2I(s)/I(0)
Uncharacterized hydrolase SAUSA300_2518 Kratky plot 1.104 0 √3 sRg
p(r)
Uncharacterized hydrolase SAUSA300_2518 pair distance distribution function Rg: 3.1 nm 0 Dmax: 10 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Uncharacterized hydrolase SAUSA300_2518 SREFLEX model
log I(s)
 s, nm-1
Uncharacterized hydrolase SAUSA300_2518 PDB (PROTEIN DATA BANK) model
log I(s)
 s, nm-1
Uncharacterized hydrolase SAUSA300_2518 DAMMIF model
Synchrotron SAXS data from solutions of carboxylic ester hydrolase FphE in 10 mM HEPES, 50 mM NaCl, 0.1% w/v NaN3, pH 7.5 were collected on the BioSAXS beam line at the Australian Synchrotron (Melbourne, Australia) using a Pilatus3 X 2M detector at a sample-detector distance of 2.5 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Co-flow in-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 1.0 mg/ml was injected at a 0.40 ml/min flow rate onto a GE Superdex 75 Increase 5/150 column at 21°C. 641 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

CAUTION: Data are over-subtracted.

Uncharacterized hydrolase SAUSA300_2518
Mol. type   Protein
Organism   Staphylococcus aureus (strain USA300)
Olig. state   Dimer
Mon. MW   31.2 kDa
 
UniProt   Q2FDS6 (1-276)
Sequence   FASTA
 
PDB ID   8T87