Unique structural and ligand-binding properties of the Staphylococcus aureus serine hydrolase FphE

Jo J, Upadhyay T, You X, Bennett J, Lee H, Bogyo M, Fellner M, Proceedings of the National Academy of Sciences 123(13) (2026) DOI

SASDX64 – Carboxylic ester hydrolase FphE

Uncharacterized hydrolase SAUSA300_2518
MWexperimental 57 kDa
MWexpected 62 kDa
VPorod 84 nm3
log I(s) 2.91×10-3 2.91×10-4 2.91×10-5 2.91×10-6
Uncharacterized hydrolase SAUSA300_2518 small angle scattering data  s, nm-1
ln I(s)
Uncharacterized hydrolase SAUSA300_2518 Guinier plot ln 2.92×10-3 Rg: 3.0 nm 0 (3.0 nm)-2 s2
(sRg)2I(s)/I(0)
Uncharacterized hydrolase SAUSA300_2518 Kratky plot 1.104 0 √3 sRg
p(r)
Uncharacterized hydrolase SAUSA300_2518 pair distance distribution function Rg: 3.1 nm 0 Dmax: 10 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Uncharacterized hydrolase SAUSA300_2518 SREFLEX model
log I(s)
 s, nm-1
Uncharacterized hydrolase SAUSA300_2518 PDB (PROTEIN DATA BANK) model
log I(s)
 s, nm-1
Uncharacterized hydrolase SAUSA300_2518 DAMMIF model
Synchrotron SAXS data from solutions of carboxylic ester hydrolase FphE in 10 mM HEPES, 50 mM NaCl, 0.1% w/v NaN3, pH 7.5 were collected on the BioSAXS beam line at the Australian Synchrotron (Melbourne, Australia) using a Pilatus3 X 2M detector at a sample-detector distance of 2.5 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Co-flow in-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 1.0 mg/ml was injected at a 0.40 ml/min flow rate onto a GE Superdex 75 Increase 5/150 column at 21°C. 641 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

CAUTION: Data are over-subtracted.

Uncharacterized hydrolase SAUSA300_2518
Mol. type   Protein
Organism   Staphylococcus aureus (strain USA300)
Olig. state   Dimer
Mon. MW   31.2 kDa
 
UniProt   Q2FDS6 (1-276)
Sequence   FASTA
 
PDB ID   8T87