Molecular insights into the structure and function of the Staphylococcus aureus fatty acid kinase.

Myers MJ, Xu Z Ryan BJ, DeMars ZR, Ridder MJ, Johnson DK, Krute CN, Flynn TS, Kashipathy MM, Battaile KP, Schnicker N, Lovell S, Freudenthal BD, Bose JL, J Biol Chem 300(12):107920 (2024) Europe PMC

SASDTW8 – Staphylococcus aureus Fatty Acid Kinase A

Uncharacterized protein SAUSA300_1119
MWexperimental 129 kDa
MWexpected 125 kDa
VPorod 219 nm3
log I(s) 2.90×100 2.90×10-1 2.90×10-2 2.90×10-3
Uncharacterized protein SAUSA300_1119 small angle scattering data  s, nm-1
ln I(s)
Uncharacterized protein SAUSA300_1119 Guinier plot ln 2.90×100 Rg: 4.3 nm 0 (4.3 nm)-2 s2
(sRg)2I(s)/I(0)
Uncharacterized protein SAUSA300_1119 Kratky plot 1.104 0 √3 sRg
p(r)
Uncharacterized protein SAUSA300_1119 pair distance distribution function Rg: 4.3 nm 0 Dmax: 16 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Uncharacterized protein SAUSA300_1119 DAMMIF model
log I(s)
 s, nm-1
Uncharacterized protein SAUSA300_1119 DAMMIF model
log I(s)
 s, nm-1
Synchrotron SAXS data from solutions of Fatty acid kinase A (FakA) in 50 mM Tris-HCl pH 7.4, 150 mM KCl, 5% glycerol, 1 mM TCEP were collected on the BioCAT 18-ID-D beamline at the Advanced Photon Source (APS), Argonne National Laboratory storage ring (Lemont, IL, USA) using a Pilatus3 X 1M detector at a sample-detector distance of 3.663 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC)-SAXS was employed. The SEC parameters were as follows: A 200 μl sample at 4.7 mg/ml was injected at a 0.60 ml/min flow rate onto a Superdex 200 Increase GL 10/300 column (GE healthcare) at 23°C. 2400 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Uncharacterized protein SAUSA300_1119 (FakA)
Mol. type   Protein
Organism   Staphylococcus aureus (strain USA300)
Olig. state   Dimer
Mon. MW   62.7 kDa
 
UniProt   Q2FHL2 (1-548)
Sequence   FASTA