| MWexperimental | 407 | kDa |
| MWexpected | 393 | kDa |
| VPorod | 491 | nm3 |
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log I(s)
1.29×10-2
1.29×10-3
1.29×10-4
1.29×10-5
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s, nm-1
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High-resolution structure of the vaccinia virus phospholipase D-fold endonuclease K4.
Gröger H Trouba C, Barbaste J, Lacroix G, Schoehn G, Burmeister WP, Tarbouriech N, J Mol Biol :169961 (2026) Europe PMCSASDZ78 – Octameric Assembly of the Phospholipase D–Like (PLD-Fold) Nuclease K4 Domain in the Vaccinia Virion Nicking-Joining Enzyme
Virion nicking-joining enzyme (octamer)|
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Fits and models
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Synchrotron SAXS
data from solutions of
Octameric Assembly of the Phospholipase D–Like (PLD-Fold) Nuclease K4 Domain in the Vaccinia Virion Nicking-Joining Enzyme
in
20 mM Tris pH 7.5, 150 mM NaCl, 0.5mM TCEP, pH 7.5
were collected
on the
SWING beam line
at the SOLEIL storage ring
(Saint-Aubin, France)
using a Eiger 4M detector
at a sample-detector distance of 2 m and
at a wavelength of λ = 0.1033 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 45.00 μl sample
at 1 mg/ml was injected at a 0.30 ml/min flow rate
onto a GE Superdex 200 Increase 5/150 column
at 20°C.
630 successive
1 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Molecular mass calculated from the Porod volume using 0.83 Da∙Å-3 obtained from the protein specific mass of 1,37 g∙cm-3. The sequence of the construct after tag-cleavage is provided. |
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