Small Angle Scattering Biological Data Bank SASBDB


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4 hits found for Widom 601 DNA sequence

SASDFX3 – Semi-synthetic nucleosome core particle (NCP)

Histone H3Histone H4Histone H2aHistone H2b147bp 601 Widom sequence experimental SAS data

Histone H3 Histone H4 Histone H2a Histone H2b 147bp 601 Widom sequence Kratky plot

Sample:	Histone H3 dimer, 31 kDa Xenopus laevis protein Histone H4 dimer, 22 kDa Xenopus laevis protein Histone H2a dimer, 28 kDa Xenopus laevis protein Histone H2b dimer, 27 kDa Xenopus laevis protein ...601 Widom sequence monomer, 45 kDa synthetic construct DNA
Buffer:	15 mM HEPES, 200 mM NaCl, pH: 7.3
Experiment:	SAXS data collected at BM29, ESRF on 2017 Dec 11

A Tail-Based Mechanism Drives Nucleosome Demethylation by the LSD2/NPAC Multimeric Complex. Cell Rep 27(2):387-399.e7 (2019)
Marabelli C, Marrocco B, Pilotto S, Chittori S, Picaud S, Marchese S, Ciossani G, Forneris F, Filippakopoulos P, Schoehn G, Rhodes D, Subramaniam S, Mattevi A

R_g^Guinier	4.4	nm
D_max	12.8	nm
Volume^Porod	353	nm³

SASDFY3 – Semi-synthetic nucleosome core particle (NCP) in complex with lysine-specific demethylase (LSD2) and the dehydrogenase domain plus linker of cytokine-like nuclear factor (NPAC delta-205)

Lysyne-specific Demethylase LSD2NPAC linker+DH (delta-205)Histone H3Histone H4Histone H2aHistone H2b147bp 601 Widom sequence experimental SAS data

Lysyne-specific Demethylase LSD2 NPAC linker+DH (delta-205) Histone H3 Histone H4 Histone H2a Histone H2b 147bp 601 Widom sequence Kratky plot

Sample:	Lysyne-specific Demethylase LSD2 monomer, 89 kDa Homo sapiens protein NPAC linker+DH (delta-205) tetramer, 150 kDa Homo sapiens protein Histone H3 dimer, 31 kDa Xenopus laevis protein Histone H4 dimer, 22 kDa Xenopus laevis protein Histone H2a dimer, 28 kDa Xenopus laevis protein Histone H2b dimer, 27 kDa Xenopus laevis protein ...601 Widom sequence monomer, 45 kDa synthetic construct DNA
Buffer:	15 mM HEPES, 200 mM NaCl, pH: 7.3
Experiment:	SAXS data collected at BM29, ESRF on 2017 Dec 11

R_g^Guinier	7.8	nm
D_max	30.4	nm
Volume^Porod	1100	nm³

SASDSX5 – ...Widom 601 DNA

Histone H3.2Histone H4Histone H2AHistone H2B 1.1Widom 601 DNA sequence experimental SAS data

Sample:	Histone H3.2 dimer, 31 kDa Xenopus laevis protein Histone H4 dimer, 23 kDa Xenopus laevis protein Histone H2A dimer, 28 kDa Xenopus laevis protein Histone H2B 1.1 dimer, 28 kDa Xenopus laevis protein Widom 601 DNA sequence dimer, 89 kDa Escherichia coli DNA
Buffer:	20 mM Tris-HCl , 50 mM KCl, 1 mM DTT, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Dec 2

BCL7 proteins, novel subunits of the mammalian SWI/SNF complex, bind the nucleosome core particle
Asgar Abbas kazrani

R_g^Guinier	4.3	nm
D_max	12.3	nm
Volume^Porod	350	nm³

SASDSY5 – Nucleosome (NCP) in complex with B cell lymphoma 7 protein family member A (BCL7A)

Isoform 2 of B-cell CLL/lymphoma 7 protein family member AHistone H3.2Histone H4Histone H2AHistone H2B 1.1Widom 601 DNA sequence experimental SAS data

Sample:	Isoform 2 of B-cell CLL/lymphoma 7 protein family member A monomer, 25 kDa Homo sapiens protein Histone H3.2 dimer, 31 kDa Xenopus laevis protein Histone H4 dimer, 23 kDa Xenopus laevis protein Histone H2A dimer, 28 kDa Xenopus laevis protein Histone H2B 1.1 dimer, 28 kDa Xenopus laevis protein Widom 601 DNA sequence dimer, 89 kDa Escherichia coli DNA
Buffer:	20 mM Tris-HCl , 50 mM KCl, 1 mM DTT, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Dec 2

BCL7 proteins, novel subunits of the mammalian SWI/SNF complex, bind the nucleosome core particle
Asgar Abbas kazrani

R_g^Guinier	4.7	nm
D_max	24.4	nm
Volume^Porod	400	nm³

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SASDFX3 – Semi-synthetic nucleosome core particle (NCP)

SASDFY3 – Semi-synthetic nucleosome core particle (NCP) in complex with lysine-specific demethylase (LSD2) and the dehydrogenase domain plus linker of cytokine-like nuclear factor (NPAC delta-205)

SASDSX5 – ...Widom 601 DNA

SASDSY5 – Nucleosome (NCP) in complex with B cell lymphoma 7 protein family member A (BCL7A)