Structural analyses of an RNA stability element interacting with poly(A)

Torabi S, Chen Y Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J, Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021) DOI

SASDKW2 – Wild type double Element for Nuclear Expression (dENE) + Poly(A)30

Wild type dENE + Poly(A)30
MWexperimental 33 kDa
MWexpected 33 kDa
log I(s) 9.15×100 9.15×10-1 9.15×10-2 9.15×10-3
Wild type dENE + Poly(A)30 small angle scattering data  s, nm-1
ln I(s)
Wild type dENE + Poly(A)30 Guinier plot ln 9.16×100 Rg: 2.9 nm 0 (2.9 nm)-2 s2
(sRg)2I(s)/I(0)
Wild type dENE + Poly(A)30 Kratky plot 1.104 0 √3 sRg
p(r)
Wild type dENE + Poly(A)30 pair distance distribution function Rg: 3.1 nm 0 Dmax: 10.6 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Wild type double Element for Nuclear Expression (dENE) + Poly(A)30 in 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH 7 were collected on the 16-ID (LiX) beam line at the National Synchrotron Light Source II (NSLS-II) storage ring (Upton, NY, USA) using a Pilatus3 S 1M detector at a sample-detector distance of 3.0 m and at a wavelength of λ = 0.092 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.66 mg/ml was measured at 10°C. 15 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Wild type dENE + Poly(A)30 (WT dENE + Poly(A)30)
Mol. type   RNA
Olig. state   Monomer
Mon. MW   32.8 kDa
Sequence   FASTA