Structural analyses of an RNA stability element interacting with poly(A)

Torabi S, Chen Y Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J, Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021) DOI

SASDKE3 – double Element for Nuclear Expression (dENE) Crystal Structure + Poly(A)20

Xtal dENE + Poly(A)20
MWexperimental 35 kDa
MWexpected 34 kDa
log I(s) 1.97×100 1.97×10-1 1.97×10-2 1.97×10-3
Xtal dENE + Poly(A)20 small angle scattering data  s, nm-1
ln I(s)
Xtal dENE + Poly(A)20 Guinier plot ln 1.97×100 Rg: 3.2 nm 0 (3.2 nm)-2 s2
(sRg)2I(s)/I(0)
Xtal dENE + Poly(A)20 Kratky plot 1.104 0 √3 sRg
p(r)
Xtal dENE + Poly(A)20 pair distance distribution function Rg: 3.4 nm 0 Dmax: 11.8 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of double Element for Nuclear Expression (dENE) Crystal Structure + Poly(A)20 in 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH 7 were collected on the G1 beam line at the Cornell High Energy Synchrotron Source (CHESS) storage ring (Ithaca, NY, USA) using a Eiger X 1M detector at a sample-detector distance of 1.7 m and at a wavelength of λ = 0.12 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.70 mg/ml was measured at 22°C. 60 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Xtal dENE + Poly(A)20 (Xta dENE + Poly(A)20)
Mol. type   RNA
Olig. state   Monomer
Mon. MW   34.5 kDa
Sequence   FASTA