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108 hits found for Pabit

SASDPU5 – Murine hepatitis virus (MHV) coronavirus putative packaging signal RNA

MHV coronavirus putative packaging signal RNA experimental SAS data
MHV coronavirus putative packaging signal RNA Kratky plot
Sample: MHV coronavirus putative packaging signal RNA monomer, 31 kDa Murine coronavirus RNA
Buffer: 70 mM KCl, 50 μM EDTA, 25 mM HEPES, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2021 Jun 10
MHV Coronavirus Packaging Signal RNA and N protein
Suzette Pabit
RgGuinier 3.9 nm
Dmax 12.0 nm
VolumePorod 58 nm3

SASDPV5 – Murine hepatitis virus (MHV) coronavirus putative packaging signal RNA (SEC-SAXS)

MHV coronavirus putative packaging signal RNA experimental SAS data
MHV coronavirus putative packaging signal RNA Kratky plot
Sample: MHV coronavirus putative packaging signal RNA monomer, 31 kDa Murine coronavirus RNA
Buffer: 70 mM KCl, 50 μM EDTA, 25 mM HEPES, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Jul 16
MHV Coronavirus Packaging Signal RNA and N protein
Suzette Pabit
RgGuinier 3.9 nm
Dmax 12.3 nm
VolumePorod 55 nm3

SASDPW5 – Murine hepatitis virus (MHV) coronavirus putative packaging signal RNA (SAXS/WAXS)

MHV coronavirus putative packaging signal RNA experimental SAS data
MHV coronavirus putative packaging signal RNA Kratky plot
Sample: MHV coronavirus putative packaging signal RNA monomer, 31 kDa Murine coronavirus RNA
Buffer: 50 mM NaCl, 50 μM EDTA, 10 mM MOPS, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2020 Sep 10
MHV Coronavirus Packaging Signal RNA and N protein
Suzette Pabit
RgGuinier 3.8 nm
Dmax 13.3 nm
VolumePorod 58 nm3

SASDPX5 – Murine hepatitis virus (MHV) coronavirus nucleocapsid protein N

Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein monomer, 50 kDa Murine coronavirus (strain … protein
Buffer: 70 mM KCl, 50 μM EDTA, 25 mM HEPES, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2021 Jun 10
MHV Coronavirus Packaging Signal RNA and N protein
Suzette Pabit
RgGuinier 5.1 nm
Dmax 11.9 nm
VolumePorod 91 nm3

SASDBS7 – Nucleosome Core Particle: 1.2 M NaCl, 50% sucrose, 50ms - 10s time resolved data

Nucleasome Core Particle with Widom 601 DNA - HISTONE H2A-H2B HeterodimerNucleasome Core Particle with Widom 601 DNA - HISTONE H3-H4 HeterodimerNucleasome Core Particle with Widom 601 DNA - dsDNA experimental SAS data
Nucleasome Core Particle with Widom 601 DNA - HISTONE H2A-H2B Heterodimer Nucleasome Core Particle with Widom 601 DNA - HISTONE H3-H4 Heterodimer Nucleasome Core Particle with Widom 601 DNA - dsDNA Kratky plot
Sample: Nucleasome Core Particle with Widom 601 DNA - HISTONE H2A-H2B Heterodimer dimer, 48 kDa Xenopus laevis protein
Nucleasome Core Particle with Widom 601 DNA - HISTONE H3-H4 Heterodimer dimer, 46 kDa Xenopus laevis protein
Nucleasome Core Particle with Widom 601 DNA - dsDNA monomer, 92 kDa Xenopus laevis DNA
Buffer: 20 mM Tris-Cl, 0.1 mM EDTA, 0.1 mM DTT, 50% sucrose, 1.2 M NaCl, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2014 Apr 14
Asymmetric unwrapping of nucleosomal DNA propagates asymmetric opening and dissociation of the histone core. Proc Natl Acad Sci U S A 114(2):334-339 (2017)
...Pabit SA, Gloss LM, Pollack L
RgGuinier 5.8 nm

SASDBT7 – Nucleosome Core Particle: 1.9 M NaCl, 50% sucrose, 50ms - 10s time resolved data

Nucleasome Core Particle with Widom 601 DNA - HISTONE H2A-H2B HeterodimerNucleasome Core Particle with Widom 601 DNA - HISTONE H3-H4 HeterodimerNucleasome Core Particle with Widom 601 DNA - dsDNA experimental SAS data
Nucleasome Core Particle with Widom 601 DNA - HISTONE H2A-H2B Heterodimer Nucleasome Core Particle with Widom 601 DNA - HISTONE H3-H4 Heterodimer Nucleasome Core Particle with Widom 601 DNA - dsDNA Kratky plot
Sample: Nucleasome Core Particle with Widom 601 DNA - HISTONE H2A-H2B Heterodimer dimer, 48 kDa Xenopus laevis protein
Nucleasome Core Particle with Widom 601 DNA - HISTONE H3-H4 Heterodimer dimer, 46 kDa Xenopus laevis protein
Nucleasome Core Particle with Widom 601 DNA - dsDNA monomer, 92 kDa Xenopus laevis DNA
Buffer: 20 mM Tris-Cl, 0.1 mM EDTA, 0.1 mM DTT, 50% sucrose, 1.9 M NaCl, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2014 Apr 14
Asymmetric unwrapping of nucleosomal DNA propagates asymmetric opening and dissociation of the histone core. Proc Natl Acad Sci U S A 114(2):334-339 (2017)
...Pabit SA, Gloss LM, Pollack L
RgGuinier 6.8 nm

SASDJV7 – pri-miR16-1 primary microRNA

primary microRNA pri-miR16-1 experimental SAS data
DAMMIF model
Sample: primary microRNA pri-miR16-1 monomer, 36 kDa Homo sapiens RNA
Buffer: 50 mM KCl, 50 mM HEPES, 5 mM DTT, 1% glycerol, pH: 7.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 12
Elucidating the Role of Microprocessor Protein DGCR8 in Bending RNA Structures Biophysical Journal (2020)
Pabit S, Chen Y, Usher E, Cook E, Pollack L, Showalter S
RgGuinier 4.4 nm
Dmax 17.2 nm
VolumePorod 74 nm3

SASDJW7 – pri-miR16-1 primary microRNA in complex with DGCR8-core protein

primary microRNA pri-miR16-1 complexed with DGCR8-core proteinMicroprocessor complex subunit DGCR8 experimental SAS data
DAMMIF model
Sample: primary microRNA pri-miR16-1 complexed with DGCR8-core protein monomer, 36 kDa Homo sapiens RNA
Microprocessor complex subunit DGCR8 monomer, 26 kDa Homo sapiens protein
Buffer: 50 mM KCl, 50 mM HEPES, 5 mM DTT, 1% glycerol, 50% sucrose, DGCR8-core, pH: 7.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 12
Elucidating the Role of Microprocessor Protein DGCR8 in Bending RNA Structures Biophysical Journal (2020)
Pabit S, Chen Y, Usher E, Cook E, Pollack L, Showalter S
RgGuinier 5.0 nm
Dmax 17.2 nm
VolumePorod 125 nm3