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7 hits found for Cerqueira

SASDMZ9 – Dockerin domain-containing protein, starch adherence system 20 (Sas20), domains 1 and 2

Dockerin domain-containing protein experimental SAS data
MULTIFOXS model
Sample: Dockerin domain-containing protein monomer, 57 kDa Ruminococcus bromii protein
Buffer: phosphate buffered saline, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Mar 28
Sas20 is a highly flexible starch-binding protein in the Ruminococcus bromii cell-surface amylosome Journal of Biological Chemistry :101896 (2022)
Cerqueira F, Photenhauer A, Doden H, Brown A, Abdel-Hamid A, Moraïs S, Bayer E, Wawrzak Z, Cann I, Ridlon J, Hopkins J, Koropatkin N
RgGuinier 5.4 nm
Dmax 20.3 nm
VolumePorod 93 nm3

SASDN22 – Dockerin domain-containing protein, starch adherence system 20 (Sas20), domain 1 with maltoheptaose

Dockerin domain-containing protein experimental SAS data
Dockerin domain-containing protein Kratky plot
Sample: Dockerin domain-containing protein monomer, 26 kDa Ruminococcus bromii protein
Buffer: phosphate buffered saline, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 14
Sas20 is a highly flexible starch-binding protein in the Ruminococcus bromii cell-surface amylosome Journal of Biological Chemistry :101896 (2022)
Cerqueira F, Photenhauer A, Doden H, Brown A, Abdel-Hamid A, Moraïs S, Bayer E, Wawrzak Z, Cann I, Ridlon J, Hopkins J, Koropatkin N
RgGuinier 2.0 nm
Dmax 7.8 nm
VolumePorod 45 nm3

SASDN32 – Dockerin domain-containing protein, starch adherence system 20 (Sas20), domain 2 with maltoheptaose

Dockerin domain-containing protein experimental SAS data
MULTIFOXS model
Sample: Dockerin domain-containing protein monomer, 26 kDa Ruminococcus bromii protein
Buffer: phosphate buffered saline, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 14
Sas20 is a highly flexible starch-binding protein in the Ruminococcus bromii cell-surface amylosome Journal of Biological Chemistry :101896 (2022)
Cerqueira F, Photenhauer A, Doden H, Brown A, Abdel-Hamid A, Moraïs S, Bayer E, Wawrzak Z, Cann I, Ridlon J, Hopkins J, Koropatkin N
RgGuinier 2.1 nm
Dmax 7.4 nm
VolumePorod 41 nm3

SASDN42 – Dockerin domain-containing protein, starch adherence system 20 (Sas20), domains 1 and 2 with maltoheptaose

Dockerin domain-containing protein experimental SAS data
Dockerin domain-containing protein Kratky plot
Sample: Dockerin domain-containing protein monomer, 26 kDa Ruminococcus bromii protein
Buffer: phosphate buffered saline, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 14
Sas20 is a highly flexible starch-binding protein in the Ruminococcus bromii cell-surface amylosome Journal of Biological Chemistry :101896 (2022)
Cerqueira F, Photenhauer A, Doden H, Brown A, Abdel-Hamid A, Moraïs S, Bayer E, Wawrzak Z, Cann I, Ridlon J, Hopkins J, Koropatkin N
RgGuinier 5.2 nm
Dmax 19.0 nm
VolumePorod 84 nm3

SASDPE2 – Starch adherence system protein 6 (Sas6)

Dockerin type I repeat experimental SAS data
MULTIFOXS model
Sample: Dockerin type I repeat monomer, 69 kDa Ruminococcus bromii protein
Buffer: phosphate buffered saline, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 14
The Ruminococcus bromii amylosome protein Sas6 binds single and double helical α-glucan structures in starch. Nat Struct Mol Biol (2024)
...Cerqueira FM, Armbruster KM, Mareček F, Chen T, Wawrzak Z, Hopkins JB, Vander Kooi CW, Janeček Š, Ruotolo BT, Koropatkin NM
RgGuinier 3.0 nm
Dmax 9.0 nm
VolumePorod 97 nm3

SASDMX9 – Dockerin domain-containing protein, starch adherence system 20 (Sas20), domain 1

Dockerin domain-containing protein experimental SAS data
MULTIFOXS model
Sample: Dockerin domain-containing protein monomer, 26 kDa Ruminococcus bromii protein
Buffer: phosphate buffered saline, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Mar 28
Sas20 is a highly flexible starch-binding protein in the Ruminococcus bromii cell-surface amylosome Journal of Biological Chemistry :101896 (2022)
Cerqueira F, Photenhauer A, Doden H, Brown A, Abdel-Hamid A, Moraïs S, Bayer E, Wawrzak Z, Cann I, Ridlon J, Hopkins J, Koropatkin N
RgGuinier 2.1 nm
Dmax 10.3 nm
VolumePorod 42 nm3

SASDMY9 – Dockerin domain-containing protein, starch adherence system 20 (Sas20), domain 2

Dockerin domain-containing protein experimental SAS data
MULTIFOXS model
Sample: Dockerin domain-containing protein monomer, 26 kDa Ruminococcus bromii protein
Buffer: phosphate buffered saline, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Mar 28
Sas20 is a highly flexible starch-binding protein in the Ruminococcus bromii cell-surface amylosome Journal of Biological Chemistry :101896 (2022)
Cerqueira F, Photenhauer A, Doden H, Brown A, Abdel-Hamid A, Moraïs S, Bayer E, Wawrzak Z, Cann I, Ridlon J, Hopkins J, Koropatkin N
RgGuinier 2.3 nm
Dmax 7.8 nm
VolumePorod 35 nm3