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Sample: |
Chromodomain-helicase-DNA-binding protein 1 dimer, 266 kDa Saccharomyces cerevisiae protein
169 bp DNA (145 bp Widom 601, flanked by 12bp DNA) monomer, 52 kDa DNA
...1 monomer, 14 kDa Xenopus laevis protein
...1.1 monomer, 14 kDa Xenopus laevis protein
Histone H3.2 monomer, 15 kDa Xenopus laevis protein
Histone H4 monomer, 11 kDa Xenopus laevis protein
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Buffer: |
10 mM Tris, 100 mM NaCl, 2 mM MgCl2, 0.1 mM EDTA, 1 mM DTT, 60% (w/v) sucrose, ADP-BeF3 (0.5 mM ADP, 4 mM NaF, 0.6 mM BeCl2), pH: 7.8
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Experiment: |
SAXS
data collected at ...1, Cornell High Energy Synchrotron Source (CHESS) on ...15 Oct 24
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...1 chromatin remodeler stimulates DNA unwrapping from the nucleosome.
Nucleic Acids Res 46(10):4978-4990 (2018)
Tokuda JM, Ren R, Levendosky RF, Tay RJ, Yan M, Pollack L, Bowman GD
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RgGuinier |
5.3 |
nm |
Dmax |
16.5 |
nm |
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