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7 hits found for GTPase Obg

SASDBS6 – ...ObgE from E.coli (ObgE_FL)

GTPase ObgE/CgtA experimental SAS data
Apo form of full length ObgE from E.coli (ObgE_FL) Rg histogram
Sample: GTPase ObgE/CgtA monomer, 44 kDa Escherichia coli protein
Buffer: 20 mM Hepes , 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Jun 17
...ObgE, a central regulator of bacterial persistence. J Biol Chem 292(14):5871-5883 (2017)
Gkekas S, Singh RK, Shkumatov AV, Messens J, Fauvart M, Verstraeten N, Michiels J, Versées W
RgGuinier 3.7 nm
Dmax 18.1 nm
VolumePorod 102 nm3

SASDBT6 – ...ObgE from E.coli (ObgE_FL with GppNHp)

GTPase ObgE/CgtA experimental SAS data
DAMMIN model
Sample: GTPase ObgE/CgtA monomer, 44 kDa Escherichia coli protein
Buffer: 20 mM Hepes, 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, 400 µM GppNHp, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Jun 17
...ObgE, a central regulator of bacterial persistence. J Biol Chem 292(14):5871-5883 (2017)
Gkekas S, Singh RK, Shkumatov AV, Messens J, Fauvart M, Verstraeten N, Michiels J, Versées W
RgGuinier 3.7 nm
Dmax 14.5 nm
VolumePorod 90 nm3

SASDBU6 – ...ObgE from E.coli (ObgE_340)

GTPase ObgE/CgtA experimental SAS data
Apo form of the C-terminal deletion mutant of ObgE from E.coli (ObgE_340) Rg histogram
Sample: GTPase ObgE/CgtA monomer, 39 kDa Escherichia coli protein
Buffer: 20 mM Hepes , 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Mar 12
...ObgE, a central regulator of bacterial persistence. J Biol Chem 292(14):5871-5883 (2017)
Gkekas S, Singh RK, Shkumatov AV, Messens J, Fauvart M, Verstraeten N, Michiels J, Versées W
RgGuinier 3.1 nm
Dmax 11.6 nm
VolumePorod 70 nm3

SASDBV6 – ...ObgE from E.coli (ObgE_340 with GppNHp)

GTPase ObgE/CgtA experimental SAS data
DAMMIN model
Sample: GTPase ObgE/CgtA monomer, 39 kDa Escherichia coli protein
Buffer: 20 mM Hepes, 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, 400 µM GppNHp, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Mar 12
...ObgE, a central regulator of bacterial persistence. J Biol Chem 292(14):5871-5883 (2017)
Gkekas S, Singh RK, Shkumatov AV, Messens J, Fauvart M, Verstraeten N, Michiels J, Versées W
RgGuinier 3.1 nm
Dmax 11.4 nm
VolumePorod 72 nm3

SASDQ78 – ...ObgE GTPase complex

Uncharacterized protein YbiBGTPase Obg experimental SAS data
Uncharacterized protein YbiB GTPase Obg Kratky plot
Sample: Uncharacterized protein YbiB dimer, 75 kDa Escherichia coli (strain … protein
GTPase Obg dimer, 91 kDa Escherichia coli (strain … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 2 mM DTT, 5% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Oct 24
...GTPase ObgE. Nucleic Acids Res (2023)
Deckers B, Vercauteren S, De Kock V, Martin C, Lazar T, Herpels P, Dewachter L, Verstraeten N, Peeters E, Ballet S, Michiels J, Galicia C, Versées W
RgGuinier 4.7 nm
Dmax 18.6 nm
VolumePorod 291 nm3

SASDBB9 – ...ObgE from E.coli (ObgE_FL with GDP)

GTPase ObgE/CgtA experimental SAS data
DAMMIN model
Sample: GTPase ObgE/CgtA monomer, 44 kDa Escherichia coli protein
Buffer: 20 mM Hepes, 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, 400 uM GDP, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Jun 17
...ObgE, a central regulator of bacterial persistence. J Biol Chem 292(14):5871-5883 (2017)
Gkekas S, Singh RK, Shkumatov AV, Messens J, Fauvart M, Verstraeten N, Michiels J, Versées W
RgGuinier 3.5 nm
Dmax 16.0 nm
VolumePorod 94673 nm3

SASDBC9 – ...ObgE from E.coli (ObgE_340 with GDP)

GTPase ObgE/CgtA experimental SAS data
DAMMIN model
Sample: GTPase ObgE/CgtA monomer, 39 kDa Escherichia coli protein
Buffer: 20 mM Hepes, 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, 400 uM GDP, pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, on 2015 Feb 1
...ObgE, a central regulator of bacterial persistence. J Biol Chem 292(14):5871-5883 (2017)
Gkekas S, Singh RK, Shkumatov AV, Messens J, Fauvart M, Verstraeten N, Michiels J, Versées W
RgGuinier 3.0 nm
Dmax 11.0 nm
VolumePorod 70673 nm3