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7 hits found for Shih

SASDK82 – Full-length nonstructural protein 2 (nsP2) of Chikungunya virus (CHIKV)

NsP2 protein experimental SAS data
CORAL model
Sample: NsP2 protein monomer, 90 kDa Chikungunya virus protein
Buffer: 20 mM Hepes pH 7.4, 150 mM NaCl, 1 mM DTT, 5% glycerol, pH: 7.4
Experiment: SAXS data collected at 23A, Taiwan Photon Source, NSRRC on 2019 Aug 10
Inter-domain Flexibility of Chikungunya Virus nsP2 Helicase-Protease Differentially Influences Viral RNA Replication and Infectivity. J Virol (2020)
...Shih O, Utt A, Goh WY, Lian BJ, Chen MW, Jeng US, Merits A, Luo D
RgGuinier 3.9 nm
Dmax 12.0 nm
VolumePorod 134 nm3

SASDC83 – Dimeric apoptosis regulator BAX (Bcl-2 associated X)

Apoptosis regulator BAX (Bcl-2 associated X) experimental SAS data
CORAL model
Sample: Apoptosis regulator BAX (Bcl-2 associated X) dimer, 42 kDa Homo sapiens protein
Buffer: 20mM sodium phosphate 100mM NaCl, pH: 8
Experiment: SAXS data collected at 23A, Taiwan Photon Source, NSRRC on 2015 May 28
Oligomerization process of Bcl-2 associated X protein revealed from intermediate structures in solution. Phys Chem Chem Phys 19(11):7947-7954 (2017)
Shih O, Yeh YQ, Liao KF, Sung TC, Chiang YW, Jeng US
RgGuinier 3.0 nm
Dmax 9.5 nm
VolumePorod 86 nm3

SASDC93 – Tetrameric apoptosis regulator BAX (Bcl-2 associated X)

Apoptosis regulator BAX (Bcl-2 associated X) experimental SAS data
SASREF model
Sample: Apoptosis regulator BAX (Bcl-2 associated X) tetramer, 85 kDa Homo sapiens protein
Buffer: 20mM sodium phosphate 100mM NaCl, pH: 8
Experiment: SAXS data collected at 23A, Taiwan Photon Source, NSRRC on 2015 May 28
Oligomerization process of Bcl-2 associated X protein revealed from intermediate structures in solution. Phys Chem Chem Phys 19(11):7947-7954 (2017)
Shih O, Yeh YQ, Liao KF, Sung TC, Chiang YW, Jeng US
RgGuinier 3.6 nm
Dmax 12.0 nm
VolumePorod 180 nm3

SASDS57 – Heterotrimeric membrane protein complex FtsB-FtsL-FtsQ

Cell division protein FtsBCell division protein FtsLCell division protein FtsQ experimental SAS data
DAMMIN model
Sample: Cell division protein FtsB hexamer, 70 kDa Escherichia coli (strain … protein
Cell division protein FtsL hexamer, 82 kDa Escherichia coli (strain … protein
Cell division protein FtsQ hexamer, 189 kDa Escherichia coli (strain … protein
Buffer: 20 mM HEPES pH 7.5, 200 mM NaCl, 0.2% of Cymal-5, pH: 7.5
Experiment: SAXS data collected at 13A, Taiwan Photon Source, NSRRC on 2022 Sep 23
Structure of the heterotrimeric membrane protein complex FtsB-FtsL-FtsQ of the bacterial divisome Nature Communications 14(1) (2023)
...Shih O, Jeng U, Huang C, Shih Y, Ma C
RgGuinier 5.3 nm
Dmax 16.5 nm
VolumePorod 421 nm3

SASDRL8 – Bovine serum albumin

Albumin experimental SAS data
Albumin Kratky plot
Sample: Albumin monomer, 66 kDa Bos taurus protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at 13A, Taiwan Photon Source, NSRRC on 2022 Jun 7
NSRRC TPS13A standard protein archive
Orion Shih
RgGuinier 2.9 nm
Dmax 8.9 nm
VolumePorod 84 nm3

SASDRM8 – Lysozyme C (Gallus gallus)

Lysozyme C experimental SAS data
Lysozyme C Kratky plot
Sample: Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer: 40 mM sodium acetate, 50 mM NaCl, pH: 4
Experiment: SAXS data collected at 13A, Taiwan Photon Source, NSRRC on 2021 Apr 2
NSRRC TPS13A standard protein archive
Orion Shih
RgGuinier 1.4 nm
Dmax 4.6 nm
VolumePorod 13 nm3

SASDRN8 – α/β Hemoglobin (human)

Hemoglobin subunit alphaHemoglobin subunit beta experimental SAS data
Hemoglobin subunit alpha Hemoglobin subunit beta Kratky plot
Sample: Hemoglobin subunit alpha dimer, 31 kDa Homo sapiens protein
Hemoglobin subunit beta dimer, 32 kDa Homo sapiens protein
Buffer: phosphate buffered saline, pH: 7.5
Experiment: SAXS data collected at 13A, Taiwan Photon Source, NSRRC on 2021 Oct 7
NSRRC TPS13A standard protein archive
Orion Shih
RgGuinier 2.5 nm
Dmax 9.2 nm
VolumePorod 97 nm3