Small Angle Scattering Biological Data Bank SASBDB

SASBDB entries for UniProt ID:

SASDYV4 – Heat shock cognate 71 kDa protein (Hsc70) T204A mutant with ADP (1 mg/ml)

UniProt ID: P11142 (1-646) Heat shock cognate 71 kDa protein

Heat shock cognate 71 kDa protein experimental SAS data

Heat shock cognate 71 kDa protein Kratky plot

Sample:	Heat shock cognate 71 kDa protein, 71 kDa protein
Buffer:	20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1mM TCEP, 1mM ADP, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6

Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A

R_g^Guinier	5.2	nm
D_max	22.0	nm
Volume^Porod	252	nm³

SASDYW4 – Heat shock cognate 71 kDa protein (Hsc70) T204A with ADP (2 mg/ml)

UniProt ID: P11142 (1-646) Heat shock cognate 71 kDa protein

Sample:	Heat shock cognate 71 kDa protein, 71 kDa protein
Buffer:	20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1mM TCEP, 1mM ADP, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6

Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A

R_g^Guinier	5.5	nm
D_max	22.9	nm
Volume^Porod	291	nm³

SASDYX4 – Heat shock cognate 71 kDa protein (Hsc70) T204A with ADP (3 mg/ml)

UniProt ID: P11142 (1-646) Heat shock cognate 71 kDa protein

Sample:	Heat shock cognate 71 kDa protein, 71 kDa protein
Buffer:	20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1mM TCEP, 1mM ADP, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6

Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A

R_g^Guinier	5.7	nm
D_max	23.8	nm
Volume^Porod	340	nm³

SASDYY4 – Heat shock cognate 71 kDa protein (Hsc70) T204A with ADP (4 mg/ml)

UniProt ID: P11142 (1-646) Heat shock cognate 71 kDa protein

Sample:	Heat shock cognate 71 kDa protein, 71 kDa protein
Buffer:	20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1mM TCEP, 1mM ADP, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6

Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A

R_g^Guinier	5.7	nm
D_max	23.5	nm
Volume^Porod	317	nm³

SASDYZ4 – Heat shock cognate 71 kDa protein (Hsc70) T204A with ADP (7 mg/ml)

UniProt ID: P11142 (1-646) Heat shock cognate 71 kDa protein

Sample:	Heat shock cognate 71 kDa protein, 71 kDa protein
Buffer:	20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1mM TCEP, 1mM ADP, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6

Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A

R_g^Guinier	5.9	nm
D_max	25.5	nm
Volume^Porod	334	nm³

SASDY25 – Heat shock cognate 71 kDa protein (Hsc70) T204A APO (0.5 mg/ml)

UniProt ID: P11142 (1-646) Heat shock cognate 71 kDa protein

Sample:	Heat shock cognate 71 kDa protein, 71 kDa protein
Buffer:	20 mM HEPES, 150 mM KCl, 10 mM M gCl2, 1mM TCEP, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6

Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A

R_g^Guinier	5.6	nm
D_max	24.0	nm
Volume^Porod	324	nm³

SASDY35 – Heat shock cognate 71 kDa protein (Hsc70) T204A apo (2 mg/ml)

UniProt ID: P11142 (1-646) Heat shock cognate 71 kDa protein

Sample:	Heat shock cognate 71 kDa protein, 71 kDa protein
Buffer:	20 mM HEPES, 150 mM KCl, 10 mM M gCl2, 1mM TCEP, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6

Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A

R_g^Guinier	5.8	nm
D_max	25.0	nm
Volume^Porod	351	nm³

SASDY45 – Heat shock cognate 71 kDa protein (Hsc70) T204A apo (3 mg/ml)

UniProt ID: P11142 (1-646) Heat shock cognate 71 kDa protein

Sample:	Heat shock cognate 71 kDa protein, 71 kDa protein
Buffer:	20 mM HEPES, 150 mM KCl, 10 mM M gCl2, 1mM TCEP, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6

Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A

R_g^Guinier	5.6	nm
D_max	25.0	nm
Volume^Porod	348	nm³

SASDY55 – Heat shock cognate 71 kDa protein (Hsc70) T204A apo (4 mg/ml)

UniProt ID: P11142 (1-646) Heat shock cognate 71 kDa protein

Sample:	Heat shock cognate 71 kDa protein, 71 kDa protein
Buffer:	20 mM HEPES, 150 mM KCl, 10 mM M gCl2, 1mM TCEP, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6

Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A

R_g^Guinier	5.7	nm
D_max	24.5	nm
Volume^Porod	351	nm³

SASDY65 – Heat shock cognate 71 kDa protein (Hsc70) T204A apo (5 mg/ml)

UniProt ID: P11142 (1-646) Heat shock cognate 71 kDa protein

Sample:	Heat shock cognate 71 kDa protein, 71 kDa protein
Buffer:	20 mM HEPES, 150 mM KCl, 10 mM M gCl2, 1mM TCEP, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6

Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A

R_g^Guinier	5.7	nm
D_max	24.7	nm
Volume^Porod	352	nm³

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