Small Angle Scattering Biological Data Bank SASBDB

SASBDB entries for UniProt ID: F2L613

SASDHG9 – Trehalose transferase (TreT) from Thermoproteus uzoniensis - apo form

UniProt ID: F2L613 (1-401) Trehalose transferase (Trehalose phosphorylase/synthase)

Trehalose transferase (Trehalose phosphorylase/synthase) experimental SAS data

Sample:	Trehalose transferase (Trehalose phosphorylase/synthase) monomer, 47 kDa Thermoproteus uzoniensis protein
Buffer:	50 mM HEPES, 100 NaCl, 4 mM DTT,, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Sep 27

Anomeric Selectivity of Trehalose Transferase with Rare L-Sugars ACS Catalysis (2020)
Mestrom L, Marsden S, van der Eijk H, Laustsen J, Jeffries C, Svergun D, Hagedoorn P, Bento I, Hanefeld U

R_g^Guinier	2.5	nm
D_max	8.8	nm
Volume^Porod	77	nm³

SASDHH9 – Trehalose transferase (TreT) from Thermoproteus uzoniensis - with magnesium

UniProt ID: F2L613 (1-401) Trehalose transferase (Trehalose phosphorylase/synthase)

Sample:	Trehalose transferase (Trehalose phosphorylase/synthase) monomer, 47 kDa Thermoproteus uzoniensis protein
Buffer:	50 mM HEPES, 100 NaCl, 4 mM DTT, 20 mM MgCl2, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Sep 27

Anomeric Selectivity of Trehalose Transferase with Rare L-Sugars ACS Catalysis (2020)
Mestrom L, Marsden S, van der Eijk H, Laustsen J, Jeffries C, Svergun D, Hagedoorn P, Bento I, Hanefeld U

R_g^Guinier	2.4	nm
D_max	8.5	nm
Volume^Porod	76	nm³

SASDHJ9 – Trehalose transferase (TreT) from Thermoproteus uzoniensis - with magnesium and trehalose

UniProt ID: F2L613 (1-401) Trehalose transferase (Trehalose phosphorylase/synthase)

Sample:	Trehalose transferase (Trehalose phosphorylase/synthase) monomer, 47 kDa Thermoproteus uzoniensis protein
Buffer:	50 mM HEPES, 100 NaCl, 4 mM DTT, 20 mM MgCl2, 1 mM trehalose, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Sep 27

Anomeric Selectivity of Trehalose Transferase with Rare L-Sugars ACS Catalysis (2020)
Mestrom L, Marsden S, van der Eijk H, Laustsen J, Jeffries C, Svergun D, Hagedoorn P, Bento I, Hanefeld U

R_g^Guinier	2.4	nm
D_max	8.3	nm
Volume^Porod	76	nm³

SASDHK9 – Trehalose transferase (TreT) from Thermoproteus uzoniensis - with magnesium and UDP-glucose

UniProt ID: F2L613 (1-401) Trehalose transferase (Trehalose phosphorylase/synthase)

Sample:	Trehalose transferase (Trehalose phosphorylase/synthase) monomer, 47 kDa Thermoproteus uzoniensis protein
Buffer:	50 mM HEPES, 100 NaCl, 4 mM DTT, 20 mM MgCl2, 1 mM UDP-glucose, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Sep 27

Anomeric Selectivity of Trehalose Transferase with Rare L-Sugars ACS Catalysis (2020)
Mestrom L, Marsden S, van der Eijk H, Laustsen J, Jeffries C, Svergun D, Hagedoorn P, Bento I, Hanefeld U

R_g^Guinier	2.4	nm
D_max	8.5	nm
Volume^Porod	73	nm³