Small Angle Scattering Biological Data Bank SASBDB

SASBDB entries for UniProt ID: H9N289

SASDFD7 – Pseudomonas putida CBB5 NdmAB complex - static

UniProt ID: H9N289 (None-None) Methylxanthine N1-demethylase NdmA

UniProt ID: H9N290 (None-None) Methylxanthine N3-demethylase NdmB

Methylxanthine N1-demethylase NdmAMethylxanthine N3-demethylase NdmB experimental SAS data

Sample:	Methylxanthine N1-demethylase NdmA trimer, 127 kDa Pseudomonas putida protein Methylxanthine N3-demethylase NdmB trimer, 129 kDa Pseudomonas putida protein
Buffer:	20 mM HEPES 150 mM NaCl 2 mM TCEP 10% v/v glycerol, pH: 7.5
Experiment:	SAXS data collected at 4C, Pohang Accelerator Laboratory on 2018 Jul 27

Structural and Mechanistic Insights into Caffeine Degradation by the Bacterial N-Demethylase Complex. J Mol Biol 431(19):3647-3661 (2019)
Kim JH, Kim BH, Brooks S, Kang SY, Summers RM, Song HK

R_g^Guinier	4.5	nm
D_max	12.3	nm

SASDFE7 – Pseudomonas putida CBB5 mCherry-NdmA/NdmB complex - static

UniProt ID: H9N289 (None-None) Methylxanthine N1-demethylase NdmA

UniProt ID: H9N290 (None-None) Methylxanthine N3-demethylase NdmB

Sample:	Methylxanthine N1-demethylase NdmA trimer, 207 kDa Pseudomonas putida protein Methylxanthine N3-demethylase NdmB trimer, 129 kDa Pseudomonas putida protein
Buffer:	20 mM HEPES 150 mM NaCl 2 mM TCEP 10% v/v glycerol, pH: 7.5
Experiment:	SAXS data collected at 4C, Pohang Accelerator Laboratory on 2018 Jul 27

Structural and Mechanistic Insights into Caffeine Degradation by the Bacterial N-Demethylase Complex. J Mol Biol 431(19):3647-3661 (2019)
Kim JH, Kim BH, Brooks S, Kang SY, Summers RM, Song HK

R_g^Guinier	5.4	nm
D_max	13.8	nm

SASDFF7 – Pseudomonas putida CBB5 NdmA hexamer

UniProt ID: H9N289 (None-None) Methylxanthine N1-demethylase NdmA

Methylxanthine N1-demethylase NdmA experimental SAS data

Sample:	Methylxanthine N1-demethylase NdmA hexamer, 254 kDa Pseudomonas putida protein
Buffer:	20 mM HEPES 150 mM NaCl 2 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2018 May 21

Structural and Mechanistic Insights into Caffeine Degradation by the Bacterial N-Demethylase Complex. J Mol Biol 431(19):3647-3661 (2019)
Kim JH, Kim BH, Brooks S, Kang SY, Summers RM, Song HK

R_g^Guinier	4.2	nm
D_max	11.0	nm

SASDFH7 – Pseudomonas putida CBB5 NdmAB complex

UniProt ID: H9N289 (None-None) Methylxanthine N1-demethylase NdmA

UniProt ID: H9N290 (None-None) Methylxanthine N3-demethylase NdmB

Sample:	Methylxanthine N1-demethylase NdmA trimer, 127 kDa Pseudomonas putida protein Methylxanthine N3-demethylase NdmB trimer, 129 kDa Pseudomonas putida protein
Buffer:	20 mM HEPES 150 mM NaCl 2 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2018 May 21

Structural and Mechanistic Insights into Caffeine Degradation by the Bacterial N-Demethylase Complex. J Mol Biol 431(19):3647-3661 (2019)
Kim JH, Kim BH, Brooks S, Kang SY, Summers RM, Song HK

R_g^Guinier	4.2	nm
D_max	10.9	nm

SASDFJ7 – Pseudomonas putida CBB5 mCherry-NdmA/ECFP-NdmB complex - static

UniProt ID: H9N289 (None-None) Methylxanthine N1-demethylase NdmA

UniProt ID: H9N290 (None-None) Methylxanthine N3-demethylase NdmB

Sample:	Methylxanthine N1-demethylase NdmA trimer, 207 kDa Pseudomonas putida protein Methylxanthine N3-demethylase NdmB trimer, 208 kDa Pseudomonas putida protein
Buffer:	20 mM HEPES 150 mM NaCl 2 mM TCEP 10% v/v glycerol, pH: 7.5
Experiment:	SAXS data collected at 4C, Pohang Accelerator Laboratory on 2018 Jul 27

Structural and Mechanistic Insights into Caffeine Degradation by the Bacterial N-Demethylase Complex. J Mol Biol 431(19):3647-3661 (2019)
Kim JH, Kim BH, Brooks S, Kang SY, Summers RM, Song HK

R_g^Guinier	5.6	nm
D_max	19.1	nm