SASBDB entries for UniProt ID: O08967

SASDCK7 – Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-399)

UniProt ID: O08967 (63-399) Cytohesin-3

Cytohesin-3 experimental SAS data
DAMMIF model
Sample: Cytohesin-3 monomer, 40 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 13
Structural Dynamics Control Allosteric Activation of Cytohesin Family Arf GTPase Exchange Factors. Structure 26(1):106-117.e6 (2018)
Malaby AW, Das S, Chakravarthy S, Irving TC, Bilsel O, Lambright DG
RgGuinier 2.8 nm
Dmax 11.2 nm
VolumePorod 61 nm3

SASDCL7 – Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-390)

UniProt ID: O08967 (63-399) Cytohesin-3

Cytohesin-3 experimental SAS data
Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-390) Rg histogram
Sample: Cytohesin-3 monomer, 40 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.001 mM inositol 1,3,4,5-tetrakisphosphate, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Mar 21
Structural Dynamics Control Allosteric Activation of Cytohesin Family Arf GTPase Exchange Factors. Structure 26(1):106-117.e6 (2018)
Malaby AW, Das S, Chakravarthy S, Irving TC, Bilsel O, Lambright DG
RgGuinier 2.8 nm
Dmax 9.3 nm
VolumePorod 64 nm3

SASDG64 – Autoinhibited dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-399) with Inositol 1,3,4,5-tetrakis phosphate (DAMMIF and GASBOR models)

UniProt ID: O08967 (14-399) Cytohesin-3

Cytohesin-3 experimental SAS data
DAMMIF model
Sample: Cytohesin-3 dimer, 93 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15
Structural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes
David Lambright
RgGuinier 5.5 nm
Dmax 26.0 nm
VolumePorod 194 nm3

SASDG94 – Autoinhibited dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-399) with Inositol 1,3,4,5-tetrakis phosphate (antiparallel CORAL and MultiFoXS models)

UniProt ID: O08967 (14-399) Cytohesin-3

Cytohesin-3 experimental SAS data
CORAL model
Sample: Cytohesin-3 dimer, 93 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15
Structural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes
David Lambright
RgGuinier 5.5 nm
Dmax 26.0 nm
VolumePorod 194 nm3

SASDGA4 – Autoinhibited dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-399) with Inositol 1,3,4,5-tetrakis phosphate (parallel CORAL and MultiFoXS models)

UniProt ID: O08967 (14-399) Cytohesin-3

Cytohesin-3 experimental SAS data
CORAL model
Sample: Cytohesin-3 dimer, 93 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15
Structural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes
David Lambright
RgGuinier 5.5 nm
Dmax 26.0 nm
VolumePorod 194 nm3

SASDG74 – Active dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-390) with Inositol 1,3,4,5-tetrakis phosphate (DAMMIF and GASBOR models)

UniProt ID: O08967 (14-390) Cytohesin-3

Cytohesin-3 experimental SAS data
DAMMIF model
Sample: Cytohesin-3 dimer, 90 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15
Structural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes
David Lambright
RgGuinier 5.3 nm
Dmax 25.7 nm
VolumePorod 168 nm3

SASDGB4 – Active dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-390) with Inositol 1,3,4,5-tetrakis phosphate (antiparallel CORAL and MultiFoXS models)

UniProt ID: O08967 (14-390) Cytohesin-3

Cytohesin-3 experimental SAS data
CORAL model
Sample: Cytohesin-3 dimer, 90 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15
Structural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes
David Lambright
RgGuinier 5.1 nm
Dmax 25.7 nm
VolumePorod 168 nm3

SASDGC4 – Active dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-390) with Inositol 1,3,4,5-tetrakis phosphate (parallel CORAL and MultiFoXS models)

UniProt ID: O08967 (14-390) Cytohesin-3

Cytohesin-3 experimental SAS data
CORAL model
Sample: Cytohesin-3 dimer, 90 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15
Structural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes
David Lambright
RgGuinier 5.1 nm
Dmax 25.7 nm
VolumePorod 168 nm3