SASBDB entries for UniProt ID: O95786

SASDB78 – Probable ATP-dependent RNA helicase DDX58 (Full-length RIG-I)

UniProt ID: O95786 (1-925) Probable ATP-dependent RNA helicase DDX58
Probable ATP-dependent RNA helicase DDX58 experimental SAS data
BUNCH model
Sample: Probable ATP-dependent RNA helicase DDX58 monomer, 108 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2012 Apr 6
Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis. Nucleic Acids Res 46(6):3169-3186 (2018)
Shah N, Beckham SA, Wilce JA, Wilce MCJ
RgGuinier 4.3 nm
Dmax 14.0 nm
VolumePorod 186 nm3

SASDB88 – Probable ATP-dependent RNA helicase DDX58 (Full-length RIG-I) plus ADP-AlFx

UniProt ID: O95786 (1-925) Probable ATP-dependent RNA helicase DDX58
Probable ATP-dependent RNA helicase DDX58 experimental SAS data
EOM/RANCH model
Sample: Probable ATP-dependent RNA helicase DDX58 monomer, 108 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, 2mM ADP-AlFx, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 Nov 20
Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis. Nucleic Acids Res 46(6):3169-3186 (2018)
Shah N, Beckham SA, Wilce JA, Wilce MCJ
RgGuinier 4.2 nm
Dmax 15.6 nm
VolumePorod 190 nm3

SASDB98 – Probable ATP-dependent RNA helicase DDX58 (Full-length RIG-I) plus bound 10mer hairpin dsRNA

UniProt ID: O95786 (1-925) Probable ATP-dependent RNA helicase DDX58

UniProt ID: (1-24) 5´ppp 10mer hairpin dsRNA
Probable ATP-dependent RNA helicase DDX585´ppp 10mer hairpin dsRNA experimental SAS data
EOM/RANCH model
Sample: Probable ATP-dependent RNA helicase DDX58 monomer, 108 kDa Homo sapiens protein
5´ppp 10mer hairpin dsRNA monomer, 8 kDa RNA
Buffer: 25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 May 29
Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis. Nucleic Acids Res 46(6):3169-3186 (2018)
Shah N, Beckham SA, Wilce JA, Wilce MCJ
RgGuinier 4.1 nm
Dmax 16.1 nm
VolumePorod 160 nm3

SASDBA8 – Probable ATP-dependent RNA helicase DDX58 (Full-length RIG-I) plus 10mer hairpin dsRNA /AMP-PNP

UniProt ID: O95786 (1-925) Probable ATP-dependent RNA helicase DDX58

UniProt ID: (1-24) 5´ppp 10mer hairpin dsRNA
Probable ATP-dependent RNA helicase DDX585´ppp 10mer hairpin dsRNA experimental SAS data
EOM/RANCH model
Sample: Probable ATP-dependent RNA helicase DDX58 monomer, 108 kDa Homo sapiens protein
5´ppp 10mer hairpin dsRNA monomer, 8 kDa RNA
Buffer: 25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, 0.5 mM AMP-PNP, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 May 28
Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis. Nucleic Acids Res 46(6):3169-3186 (2018)
Shah N, Beckham SA, Wilce JA, Wilce MCJ
RgGuinier 4.2 nm
Dmax 17.0 nm
VolumePorod 163 nm3

SASDBB8 – Probable ATP-dependent RNA helicase DDX58 (Full-length RIG-I) plus 10mer hairpin dsRNA and ADP-AlFx

UniProt ID: O95786 (1-925) Probable ATP-dependent RNA helicase DDX58

UniProt ID: (1-24) 5´ppp 10mer hairpin dsRNA
Probable ATP-dependent RNA helicase DDX585´ppp 10mer hairpin dsRNA experimental SAS data
EOM/RANCH model
Sample: Probable ATP-dependent RNA helicase DDX58 monomer, 108 kDa Homo sapiens protein
5´ppp 10mer hairpin dsRNA monomer, 8 kDa RNA
Buffer: 25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, 2mM ADP-AlFx, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 Nov 20
Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis. Nucleic Acids Res 46(6):3169-3186 (2018)
Shah N, Beckham SA, Wilce JA, Wilce MCJ
RgGuinier 4.0 nm
Dmax 18.3 nm
VolumePorod 156 nm3

SASDBD8 – Probable ATP-dependent RNA helicase DDX58 (Full-length RIG-I) plus 8mer hairpin dsRNA (SEC-peak1)

UniProt ID: O95786 (1-925) Probable ATP-dependent RNA helicase DDX58

UniProt ID: (1-20) 5´ppp 8mer hairpin dsRNA
Probable ATP-dependent RNA helicase DDX585´ppp 8mer hairpin dsRNA experimental SAS data
EOM/RANCH model
Sample: Probable ATP-dependent RNA helicase DDX58 monomer, 108 kDa Homo sapiens protein
5´ppp 8mer hairpin dsRNA monomer, 6 kDa RNA
Buffer: 25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 Nov 20
Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis. Nucleic Acids Res 46(6):3169-3186 (2018)
Shah N, Beckham SA, Wilce JA, Wilce MCJ
RgGuinier 4.3 nm
Dmax 15.3 nm
VolumePorod 179 nm3

SASDBE8 – Probable ATP-dependent RNA helicase DDX58 (Full-length RIG-I) 8mer hairpin dsRNA/AMP-PNP (SEC-peak1)

UniProt ID: O95786 (1-925) Probable ATP-dependent RNA helicase DDX58

UniProt ID: (1-20) 5´ppp 8mer hairpin dsRNA
Probable ATP-dependent RNA helicase DDX585´ppp 8mer hairpin dsRNA experimental SAS data
EOM/RANCH model
Sample: Probable ATP-dependent RNA helicase DDX58 monomer, 108 kDa Homo sapiens protein
5´ppp 8mer hairpin dsRNA monomer, 6 kDa RNA
Buffer: 25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, 0.5 mM AMP-PNP, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 Apr 27
Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis. Nucleic Acids Res 46(6):3169-3186 (2018)
Shah N, Beckham SA, Wilce JA, Wilce MCJ
RgGuinier 4.1 nm
Dmax 15.0 nm
VolumePorod 188 nm3

SASDBF8 – Probable ATP-dependent RNA helicase DDX58 (Full-length RIG-I) 8mer hairpin dsRNA/ADP-AlFx (SECpeak1)

UniProt ID: O95786 (1-925) Probable ATP-dependent RNA helicase DDX58

UniProt ID: (1-20) 5´ppp 8mer hairpin dsRNA
Probable ATP-dependent RNA helicase DDX585´ppp 8mer hairpin dsRNA experimental SAS data
EOM/RANCH model
Sample: Probable ATP-dependent RNA helicase DDX58 monomer, 108 kDa Homo sapiens protein
5´ppp 8mer hairpin dsRNA monomer, 6 kDa RNA
Buffer: 25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, 2mM ADP-AlFx, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 Nov 20
Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis. Nucleic Acids Res 46(6):3169-3186 (2018)
Shah N, Beckham SA, Wilce JA, Wilce MCJ
RgGuinier 4.2 nm
Dmax 16.8 nm
VolumePorod 178 nm3

SASDBG8 – Probable ATP-dependent RNA helicase DDX58 without CARDs (Delta-CARDs RIG-I)

UniProt ID: O95786 (239-925) Probable ATP-dependent RNA helicase DDX58 (without CARDs)
Probable ATP-dependent RNA helicase DDX58 (without CARDs) experimental SAS data
BUNCH model
Sample: Probable ATP-dependent RNA helicase DDX58 (without CARDs) monomer, 80 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 May 29
Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis. Nucleic Acids Res 46(6):3169-3186 (2018)
Shah N, Beckham SA, Wilce JA, Wilce MCJ
RgGuinier 3.7 nm
Dmax 13.0 nm
VolumePorod 114 nm3

SASDBH8 – Probable ATP-dependent RNA helicase DDX58 without CARDs (Delta-CARDs RIG-I) plus ADP-AlFx

UniProt ID: O95786 (239-925) Probable ATP-dependent RNA helicase DDX58 (without CARDs)
Probable ATP-dependent RNA helicase DDX58 (without CARDs) experimental SAS data
EOM/RANCH model
Sample: Probable ATP-dependent RNA helicase DDX58 (without CARDs) monomer, 80 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, 2mM ADP-AlFx, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 Nov 20
Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis. Nucleic Acids Res 46(6):3169-3186 (2018)
Shah N, Beckham SA, Wilce JA, Wilce MCJ
RgGuinier 3.5 nm
Dmax 14.0 nm
VolumePorod 132 nm3