SASBDB entries for UniProt ID: P02649

SASDGM9 – Mutant Apolipoprotein E4 (K143A K146A) - (SEC-SAXS)

UniProt ID: P02649 (19-317) Apolipoprotein E4 (K143A K146A) mutant
Apolipoprotein E4 (K143A K146A) mutant experimental SAS data
Apolipoprotein E4 (K143A K146A) mutant Kratky plot
Sample: Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft
RgGuinier 5.8 nm
Dmax 20.2 nm

SASDGN9 – Mutant Apolipoprotein E4 (K143A K146A) bound to 1 mg/mL heparin (SEC-SAXS)

UniProt ID: None (None-None) Heparin

UniProt ID: P02649 (19-317) Apolipoprotein E4 (K143A K146A) mutant
HeparinApolipoprotein E4 (K143A K146A) mutant experimental SAS data
Heparin Apolipoprotein E4 (K143A K146A) mutant Kratky plot
Sample: Heparin monomer, 15 kDa
Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft
RgGuinier 5.7 nm
Dmax 19.8 nm

SASDGP9 – Mutant Apolipoprotein E4 (K143A K146A) bound to 1 mM Suramin (SEC-SAXS)

UniProt ID: P02649 (19-317) Apolipoprotein E4 (K143A K146A) mutant

UniProt ID: None (None-None) Suramin
Apolipoprotein E4 (K143A K146A) mutantSuramin experimental SAS data
Apolipoprotein E4 (K143A K146A) mutant Suramin Kratky plot
Sample: Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft
RgGuinier 5.4 nm
Dmax 18.3 nm

SASDGQ9 – Truncated Apolipoprotein E4 (1-191) - (SEC-SAXS)

UniProt ID: P02649 (19-209) Apolipoprotein E4 (1-191)
Apolipoprotein E4 (1-191) experimental SAS data
Apolipoprotein E4 (1-191) Kratky plot
Sample: Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft
RgGuinier 2.3 nm
Dmax 7.6 nm

SASDGR9 – Truncated Apolipoprotein E4 (1-191) bound to 1 mg/mL heparin (SEC-SAXS)

UniProt ID: None (None-None) Heparin

UniProt ID: P02649 (19-209) Apolipoprotein E4 (1-191)
HeparinApolipoprotein E4 (1-191) experimental SAS data
Heparin Apolipoprotein E4 (1-191) Kratky plot
Sample: Heparin monomer, 15 kDa
Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft
RgGuinier 4.2 nm
Dmax 16.8 nm

SASDGS9 – Truncated Apolipoprotein E4 (1-191) bound to 1 mM Suramin (SEC-SAXS)

UniProt ID: P02649 (19-209) Apolipoprotein E4 (1-191)

UniProt ID: None (None-None) Suramin
Apolipoprotein E4 (1-191)Suramin experimental SAS data
Apolipoprotein E4 (1-191) Suramin Kratky plot
Sample: Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft
RgGuinier 2.4 nm
Dmax 8.3 nm

SASDGT9 – Truncated Apolipoprotein E4 (1-191) bound to 110 µg/mL heparin

UniProt ID: None (None-None) Heparin

UniProt ID: P02649 (19-209) Apolipoprotein E4 (1-191)
HeparinApolipoprotein E4 (1-191) experimental SAS data
Heparin Apolipoprotein E4 (1-191) Kratky plot
Sample: Heparin monomer, 15 kDa
Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft
RgGuinier 2.7 nm
Dmax 8.3 nm