SASBDB entries for UniProt ID: P04925

SASDCJ2 – Solution structure of recombinant prion protein (89–230) in complex with Fab-P

UniProt ID: P04925 (23-230) Major prion protein

UniProt ID: (None-None) P-Clone Fab, Chimera
Major prion proteinP-Clone Fab, Chimera experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Major prion protein monomer, 23 kDa Mus musculus protein
P-Clone Fab, Chimera monomer, 47 kDa Homo sapiens protein
Buffer: sodium acetate buffer (20 mM sodium acetate, pH 5.1; 150 mM NaCl), pH: 5.1
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2013 Dec 5
Prion Protein-Antibody Complexes Characterized by Chromatography-Coupled Small-Angle X-Ray Scattering. Biophys J 109(4):793-805 (2015)
Carter L, Kim SJ, Schneidman-Duhovny D, Stöhr J, Poncet-Montange G, Weiss TM, Tsuruta H, Prusiner SB, Sali A
RgGuinier 3.9 nm
Dmax 14.5 nm
VolumePorod 106 nm3

SASDHV9 – Murine major prion protein PrPc 23-230

UniProt ID: P04925 (23-230) Major prion protein
Major prion protein experimental SAS data
CORAL model
Sample: Major prion protein monomer, 23 kDa Mus musculus protein
Buffer: 10mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 15
Characterization of murine prion protein
Stefano Da Vela
RgGuinier 2.8 nm
Dmax 9.9 nm
VolumePorod 43 nm3

SASDLU2 – 2:1 complex between anti prion protein monoclonal IgG 6D11 and recombinant murine prion protein

UniProt ID: P04925 (23-230) Major prion protein

UniProt ID: None (None-None) Anti-prion protein monoclonal IgG2a 6D11
Major prion proteinAnti-prion protein monoclonal IgG2a 6D11 experimental SAS data
OTHER model
Sample: Major prion protein monomer, 23 kDa Mus musculus protein
Anti-prion protein monoclonal IgG2a 6D11 monomer, 145 kDa Mus musculus protein
Buffer: phosphate buffered saline, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 15
Ligands binding to the prion protein induce its proteolytic release with therapeutic potential in neurodegenerative proteinopathies. Sci Adv 7(48):eabj1826 (2021)
Linsenmeier L, Mohammadi B, Shafiq M, Frontzek K, Bär J, Shrivastava AN, Damme M, Song F, Schwarz A, Da Vela S, Massignan T, Jung S, Correia A, Schmitz M, Puig B, Hornemann S, Zerr I, Tatzelt J, Biasini E, Saftig P, Schweizer M, Svergun D, Amin L, Mazzola F, Varani L, Thapa S, Gilch S, Schätzl H, Harris DA, Triller A, Mikhaylova M, Aguzzi A, Altmeppen HC, Glatzel M
RgGuinier 8.1 nm
Dmax 24.8 nm
VolumePorod 710 nm3