SASBDB entries for UniProt ID: P08311

SASDVK2 – Core domain of human Cathepsin-G, short truncations to both terminus (Δ22-243Δ253)

UniProt ID: P08311 (22-245) Cathepsin G
Cathepsin G experimental SAS data
PHENIX model
Sample: Cathepsin G monomer, 25 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 140 mM NaCl, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Jun 3
S. aureus Eap is a polyvalent inhibitor of neutrophil serine proteases. J Biol Chem 300(9):107627 (2024)
Mishra N, Gido CD, Herdendorf TJ, Hammel M, Hura GL, Fu ZQ, Geisbrecht BV
RgGuinier 1.9 nm
Dmax 6.9 nm
VolumePorod 27 nm3

SASDVL2 – Ternary complex consisted of Human Chatepsin G, Neutrophil elastase and S.aureus MAP domain-containing protein

UniProt ID: P08311 (22-245) Cathepsin G

UniProt ID: A0A0H3JUK5 (32-146) MAP domain-containing protein

UniProt ID: P08246 (31-249) Neutrophil elastase
Cathepsin GMAP domain-containing proteinNeutrophil elastase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Cathepsin G monomer, 25 kDa Homo sapiens protein
MAP domain-containing protein monomer, 13 kDa Staphylococcus aureus (strain … protein
Neutrophil elastase monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 140 mM NaCl, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Jun 3
S. aureus Eap is a polyvalent inhibitor of neutrophil serine proteases. J Biol Chem 300(9):107627 (2024)
Mishra N, Gido CD, Herdendorf TJ, Hammel M, Hura GL, Fu ZQ, Geisbrecht BV
RgGuinier 2.8 nm
Dmax 9.4 nm
VolumePorod 77 nm3

SASDVG2 – Staphylococcal extracellular adherence protein (Eap) bound to human cathepsin-G

UniProt ID: Q99QS1 (32-478) Protein map

UniProt ID: P08311 (22-245) Cathepsin G
Protein mapCathepsin G experimental SAS data
MULTIFOXS model
Sample: Protein map monomer, 50 kDa Staphylococcus aureus (strain … protein
Cathepsin G tetramer, 101 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 140 mM NaCl, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Jun 3
S. aureus Eap is a polyvalent inhibitor of neutrophil serine proteases. J Biol Chem 300(9):107627 (2024)
Mishra N, Gido CD, Herdendorf TJ, Hammel M, Hura GL, Fu ZQ, Geisbrecht BV
RgGuinier 5.0 nm
Dmax 20.2 nm
VolumePorod 166 nm3

SASDVP2 – Multimeric complex cosisted of Human Cathepsin G-Neutrophil elastase tetramers bound to S.aureus Protein Map Eap3 and Eap4 domains

UniProt ID: P08311 (22-245) Cathepsin G

UniProt ID: P08246 (31-249) Neutrophil elastase

UniProt ID: Q99QS1 (268-438) Eap34
Cathepsin GNeutrophil elastaseEap34 experimental SAS data
BILBOMD model
Sample: Cathepsin G tetramer, 101 kDa Homo sapiens protein
Neutrophil elastase tetramer, 93 kDa Homo sapiens protein
Eap34 dimer, 48 kDa Staphylococcus aureus (strain … protein
Buffer: 20 mM HEPES, 140 mM NaCl, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Jun 3
S. aureus Eap is a polyvalent inhibitor of neutrophil serine proteases. J Biol Chem 300(9):107627 (2024)
Mishra N, Gido CD, Herdendorf TJ, Hammel M, Hura GL, Fu ZQ, Geisbrecht BV
RgGuinier 5.4 nm
Dmax 19.4 nm
VolumePorod 500 nm3