SASBDB entries for UniProt ID: P11142

SASDL42 – Human Heat Shock Cognate 71 kDa Protein (HSPA8 / Hsc70)

UniProt ID: P11142 (1-646) Heat shock cognate 71 kDa protein
Heat shock cognate 71 kDa protein experimental SAS data
Human Heat Shock Cognate 71 kDa Protein (HSPA8 / Hsc70) Rg histogram
Sample: Heat shock cognate 71 kDa protein monomer, 71 kDa Homo sapiens protein
Buffer: 25 mM Tris HCl, 50 mM NaCl, 5 mM Sodium Phosphate, 5 mM KCl, pH: 7.5
Experiment: SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2018 May 18
Structural, thermodynamic and functional studies of human 71 kDa heat shock cognate protein (HSPA8/hHsc70) Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics :140719 (2021)
Silva N, de Camargo Rodrigues L, Dores-Silva P, Montanari C, Ramos C, Barbosa L, Borges J
RgGuinier 3.7 nm
Dmax 11.5 nm
VolumePorod 111 nm3

SASDYK4 – Heat shock cognate 71 kDa protein (Hsc70) T204A mutant with ATP (2 mg/ml)

UniProt ID: P11142 (1-646) Heat shock cognate 71 kDa protein
Heat shock cognate 71 kDa protein experimental SAS data
Heat shock cognate 71 kDa protein Kratky plot
Sample: Heat shock cognate 71 kDa protein, 71 kDa protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1 mM TCEP, 1 mM ATP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 4.1 nm
Dmax 17.5 nm
VolumePorod 143 nm3

SASDYL4 – Heat shock cognate 71 kDa protein (Hsc70) T204A mutant with ATP (3 mg/ml)

UniProt ID: P11142 (1-646) Heat shock cognate 71 kDa protein
Heat shock cognate 71 kDa protein experimental SAS data
Heat shock cognate 71 kDa protein Kratky plot
Sample: Heat shock cognate 71 kDa protein, 71 kDa protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1 mM TCEP, 1 mM ATP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 4.1 nm
Dmax 18.3 nm
VolumePorod 143 nm3

SASDYM4 – Heat shock cognate 71 kDa protein (Hsc70) T204A mutant with ATP (4 mg/ml)

UniProt ID: P11142 (1-646) Heat shock cognate 71 kDa protein
Heat shock cognate 71 kDa protein experimental SAS data
Heat shock cognate 71 kDa protein Kratky plot
Sample: Heat shock cognate 71 kDa protein, 71 kDa protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1 mM TCEP, 1 mM ATP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 4.3 nm
Dmax 18.4 nm
VolumePorod 160 nm3

SASDYN4 – Heat shock cognate 71 kDa protein (Hsc70) T204A mutant with ATP (5 mg/ml)

UniProt ID: P11142 (1-646) Heat shock cognate 71 kDa protein
Heat shock cognate 71 kDa protein experimental SAS data
Heat shock cognate 71 kDa protein Kratky plot
Sample: Heat shock cognate 71 kDa protein, 71 kDa protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1 mM TCEP, 1 mM ATP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 4.4 nm
Dmax 19.1 nm
VolumePorod 167 nm3

SASDYV4 – Heat shock cognate 71 kDa protein (Hsc70) T204A mutant with ADP (1 mg/ml)

UniProt ID: P11142 (1-646) Heat shock cognate 71 kDa protein
Heat shock cognate 71 kDa protein experimental SAS data
Heat shock cognate 71 kDa protein Kratky plot
Sample: Heat shock cognate 71 kDa protein, 71 kDa protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1mM TCEP, 1mM ADP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 5.2 nm
Dmax 22.0 nm
VolumePorod 252 nm3

SASDYW4 – Heat shock cognate 71 kDa protein (Hsc70) T204A with ADP (2 mg/ml)

UniProt ID: P11142 (1-646) Heat shock cognate 71 kDa protein
Heat shock cognate 71 kDa protein experimental SAS data
Heat shock cognate 71 kDa protein Kratky plot
Sample: Heat shock cognate 71 kDa protein, 71 kDa protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1mM TCEP, 1mM ADP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 5.5 nm
Dmax 22.9 nm
VolumePorod 291 nm3

SASDYX4 – Heat shock cognate 71 kDa protein (Hsc70) T204A with ADP (3 mg/ml)

UniProt ID: P11142 (1-646) Heat shock cognate 71 kDa protein
Heat shock cognate 71 kDa protein experimental SAS data
Heat shock cognate 71 kDa protein Kratky plot
Sample: Heat shock cognate 71 kDa protein, 71 kDa protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1mM TCEP, 1mM ADP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 5.7 nm
Dmax 23.8 nm
VolumePorod 340 nm3

SASDYY4 – Heat shock cognate 71 kDa protein (Hsc70) T204A with ADP (4 mg/ml)

UniProt ID: P11142 (1-646) Heat shock cognate 71 kDa protein
Heat shock cognate 71 kDa protein experimental SAS data
Heat shock cognate 71 kDa protein Kratky plot
Sample: Heat shock cognate 71 kDa protein, 71 kDa protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1mM TCEP, 1mM ADP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 5.7 nm
Dmax 23.5 nm
VolumePorod 317 nm3

SASDYZ4 – Heat shock cognate 71 kDa protein (Hsc70) T204A with ADP (7 mg/ml)

UniProt ID: P11142 (1-646) Heat shock cognate 71 kDa protein
Heat shock cognate 71 kDa protein experimental SAS data
Heat shock cognate 71 kDa protein Kratky plot
Sample: Heat shock cognate 71 kDa protein, 71 kDa protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1mM TCEP, 1mM ADP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 5.9 nm
Dmax 25.5 nm
VolumePorod 334 nm3