SASBDB entries for UniProt ID: P13433

SASDJT2 – DNA-directed RNA polymerase (RPO41)

UniProt ID: P13433 (30-1351) DNA-directed RNA polymerase

DNA-directed RNA polymerase experimental SAS data
DNA-directed RNA polymerase Kratky plot
Sample: DNA-directed RNA polymerase monomer, 150 kDa Saccharomyces cerevisiae protein
Buffer: 40 mM Tris, 150 mM NaCl, 5% glycerol, 1 mM DDT, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2019 Sep 25
PfKsgA1 functions as a transcription initiation factor and interacts with the N-terminal region of the mitochondrial RNA polymerase of Plasmodium falciparum. Int J Parasitol (2020)
Gupta A, Shrivastava D, Shakya AK, Gupta K, Pratap JV, Habib S
RgGuinier 4.4 nm
Dmax 12.5 nm
VolumePorod 295 nm3

SASDJU2 – DNA-directed RNA polymerase (RPO41) in complex with rRNA adenine N(6)-methyltransferase (PfKsgA1)

UniProt ID: P13433 (30-1351) DNA-directed RNA polymerase

UniProt ID: Q8ILT8 (43-381) rRNA adenine N (6) methyl transferase

DNA-directed RNA polymeraserRNA adenine N (6) methyl transferase experimental SAS data
DNA-directed RNA polymerase rRNA adenine N (6) methyl transferase Kratky plot
Sample: DNA-directed RNA polymerase monomer, 150 kDa Saccharomyces cerevisiae protein
RRNA adenine N (6) methyl transferase monomer, 39 kDa Plasmodium falciparum protein
Buffer: 40 mM Tris, 150 mM NaCl, 5% glycerol, 1 mM DDT, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2020 Jan 10
PfKsgA1 functions as a transcription initiation factor and interacts with the N-terminal region of the mitochondrial RNA polymerase of Plasmodium falciparum. Int J Parasitol (2020)
Gupta A, Shrivastava D, Shakya AK, Gupta K, Pratap JV, Habib S
RgGuinier 5.5 nm
Dmax 15.0 nm
VolumePorod 393 nm3

SASDJV2 – DNA-directed RNA polymerase (RPO41) in complex with mitochondrial transcription factor 1 (MTF1)

UniProt ID: P13433 (30-1351) DNA-directed RNA polymerase

UniProt ID: P14908 (1-341) Mitochondrial transcription factor 1

DNA-directed RNA polymeraseMitochondrial transcription factor 1 experimental SAS data
DNA-directed RNA polymerase Mitochondrial transcription factor 1 Kratky plot
Sample: DNA-directed RNA polymerase monomer, 150 kDa Saccharomyces cerevisiae protein
Mitochondrial transcription factor 1 monomer, 40 kDa Saccharomyces cerevisiae protein
Buffer: 40 mM Tris, 150 mM NaCl, 5% glycerol, 1 mM DDT, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2019 Dec 7
PfKsgA1 functions as a transcription initiation factor and interacts with the N-terminal region of the mitochondrial RNA polymerase of Plasmodium falciparum. Int J Parasitol (2020)
Gupta A, Shrivastava D, Shakya AK, Gupta K, Pratap JV, Habib S
RgGuinier 4.5 nm
Dmax 14.4 nm
VolumePorod 292 nm3