SASBDB entries for UniProt ID: P21675

SASDK52 – TAF1 tandem bromodomain

UniProt ID: P21675 (1373-1635) Transcription initiation factor II D
Transcription initiation factor II D experimental SAS data
Transcription initiation factor II D Kratky plot
Sample: Transcription initiation factor II D monomer, 31 kDa Escherichia coli protein
Buffer: 50 mM HEPES, 5% v/v ethylene glycol, 2.5% v/v DMSO and 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Oct 26
Discovery of Dual TAF1-ATR Inhibitors and Ligand-Induced Structural Changes of the TAF1 Tandem Bromodomain. J Med Chem 65(5):4182-4200 (2022)
Karim RM, Yang L, Chen L, Bikowitz MJ, Lu J, Grassie D, Shultz ZP, Lopchuk JM, Chen J, Schönbrunn E
RgGuinier 2.6 nm
Dmax 7.3 nm
VolumePorod 50 nm3

SASDK62 – TAF1 tandem bromodomain with inhibitor AZD6738

UniProt ID: P21675 (1373-1635) Transcription initiation factor II D
Transcription initiation factor II D experimental SAS data
Transcription initiation factor II D Kratky plot
Sample: Transcription initiation factor II D monomer, 31 kDa Escherichia coli protein
Buffer: 50 mM HEPES, 5% v/v ethylene glycol, 2.5% v/v DMSO and 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Oct 19
Discovery of Dual TAF1-ATR Inhibitors and Ligand-Induced Structural Changes of the TAF1 Tandem Bromodomain. J Med Chem 65(5):4182-4200 (2022)
Karim RM, Yang L, Chen L, Bikowitz MJ, Lu J, Grassie D, Shultz ZP, Lopchuk JM, Chen J, Schönbrunn E
RgGuinier 2.3 nm
Dmax 6.6 nm
VolumePorod 44 nm3

SASDK72 – TAF1 tandem bromodomain with inhibitor BAY299

UniProt ID: P21675 (1373-1635) Transcription initiation factor II D
Transcription initiation factor II D experimental SAS data
Transcription initiation factor II D Kratky plot
Sample: Transcription initiation factor II D monomer, 31 kDa Escherichia coli protein
Buffer: 50 mM HEPES, 5% v/v ethylene glycol, 2.5% v/v DMSO and 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Oct 19
Discovery of Dual TAF1-ATR Inhibitors and Ligand-Induced Structural Changes of the TAF1 Tandem Bromodomain. J Med Chem 65(5):4182-4200 (2022)
Karim RM, Yang L, Chen L, Bikowitz MJ, Lu J, Grassie D, Shultz ZP, Lopchuk JM, Chen J, Schönbrunn E
RgGuinier 3.6 nm
Dmax 12.5 nm
VolumePorod 106 nm3

SASDKK7 – Transcription initiation factor TFIID subunit 1, TAF1, tandem bromodomains (2 mg/ml)

UniProt ID: P21675 (1373-1635) Transcription initiation factor TFIID subunit 1
Transcription initiation factor TFIID subunit 1 experimental SAS data
Transcription initiation factor TFIID subunit 1 Kratky plot
Sample: Transcription initiation factor TFIID subunit 1 monomer, 31 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 150 mM NaCl, and 2% glycerol, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Sep 25
Multivalent nucleosome scaffolding by bromodomain and extraterminal domain tandem bromodomains. J Biol Chem :108289 (2025)
Olp MD, Bursch KL, Wynia-Smith SL, Nuñez R, Goetz CJ, Jackson V, Smith BC
RgGuinier 3.2 nm
Dmax 9.9 nm
VolumePorod 80 nm3

SASDKL7 – Transcription initiation factor TFIID subunit 1, TAF1, tandem bromodomains (1 mg/ml)

UniProt ID: P21675 (1373-1635) Transcription initiation factor TFIID subunit 1
Transcription initiation factor TFIID subunit 1 experimental SAS data
Transcription initiation factor TFIID subunit 1 Kratky plot
Sample: Transcription initiation factor TFIID subunit 1 monomer, 31 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 150 mM NaCl, and 2% glycerol, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Sep 25
Multivalent nucleosome scaffolding by bromodomain and extraterminal domain tandem bromodomains. J Biol Chem :108289 (2025)
Olp MD, Bursch KL, Wynia-Smith SL, Nuñez R, Goetz CJ, Jackson V, Smith BC
RgGuinier 2.9 nm
Dmax 10.6 nm
VolumePorod 65 nm3