UniProt ID: P35609 (274-746) Rod domain of α-actinin-2
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| Sample: |
Rod domain of α-actinin-2 dimer, 112 kDa Homo sapiens protein
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| Buffer: |
50 mM Tris-HCl 150 mM NaCl, pH: 7.5 |
| Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2017 Dec 5
|
Order from disorder in the sarcomere: FATZ forms a fuzzy but tight complex and phase-separated condensates with α-actinin.
Sci Adv 7(22) (2021)
Sponga A, Arolas JL, Schwarz TC, Jeffries CM, Rodriguez Chamorro A, Kostan J, Ghisleni A, Drepper F, Polyansky A, De Almeida Ribeiro E, Pedron M, Zawadzka-Kazimierczuk A, Mlynek G, Peterbauer T, Doto P, Schreiner C, Hollerl E, Mateos B, Geist L, Faulkner G, Kozminski W, Svergun DI, Warscheid B, Zagrovic B, Gautel M, Konrat R, Djinović-Carugo K
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| RgGuinier |
6.7 |
nm |
| Dmax |
27.2 |
nm |
| VolumePorod |
214 |
nm3 |
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UniProt ID: P35609 (1-894) Half dimer of α-actinin-2
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| Sample: |
Half dimer of α-actinin-2 monomer, 107 kDa Homo sapiens protein
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| Buffer: |
50 mM Tris-HCl 150 mM NaCl, pH: 7.5 |
| Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2019 May 18
|
Order from disorder in the sarcomere: FATZ forms a fuzzy but tight complex and phase-separated condensates with α-actinin.
Sci Adv 7(22) (2021)
Sponga A, Arolas JL, Schwarz TC, Jeffries CM, Rodriguez Chamorro A, Kostan J, Ghisleni A, Drepper F, Polyansky A, De Almeida Ribeiro E, Pedron M, Zawadzka-Kazimierczuk A, Mlynek G, Peterbauer T, Doto P, Schreiner C, Hollerl E, Mateos B, Geist L, Faulkner G, Kozminski W, Svergun DI, Warscheid B, Zagrovic B, Gautel M, Konrat R, Djinović-Carugo K
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| RgGuinier |
5.3 |
nm |
| Dmax |
22.0 |
nm |
| VolumePorod |
172 |
nm3 |
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UniProt ID: P35609 (274-746) Rod domain of α-actinin-2
UniProt ID: Q9NP98 (92-299) Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2)
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| Sample: |
Rod domain of α-actinin-2 dimer, 112 kDa Homo sapiens protein
Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) dimer, 43 kDa Homo sapiens protein
|
| Buffer: |
50 mM Tris-HCl 150 mM NaCl, pH: 7.5 |
| Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2019 May 18
|
Order from disorder in the sarcomere: FATZ forms a fuzzy but tight complex and phase-separated condensates with α-actinin.
Sci Adv 7(22) (2021)
Sponga A, Arolas JL, Schwarz TC, Jeffries CM, Rodriguez Chamorro A, Kostan J, Ghisleni A, Drepper F, Polyansky A, De Almeida Ribeiro E, Pedron M, Zawadzka-Kazimierczuk A, Mlynek G, Peterbauer T, Doto P, Schreiner C, Hollerl E, Mateos B, Geist L, Faulkner G, Kozminski W, Svergun DI, Warscheid B, Zagrovic B, Gautel M, Konrat R, Djinović-Carugo K
|
| RgGuinier |
7.6 |
nm |
| Dmax |
28.4 |
nm |
| VolumePorod |
371 |
nm3 |
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UniProt ID: Q9NP98 (92-299) Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2)
UniProt ID: P35609 (1-894) Half dimer of α-actinin-2
|
|
|
|
| Sample: |
Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) monomer, 22 kDa Homo sapiens protein
Half dimer of α-actinin-2 monomer, 107 kDa Homo sapiens protein
|
| Buffer: |
50 mM Tris-HCl 150 mM NaCl, pH: 7.5 |
| Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2019 May 18
|
Order from disorder in the sarcomere: FATZ forms a fuzzy but tight complex and phase-separated condensates with α-actinin.
Sci Adv 7(22) (2021)
Sponga A, Arolas JL, Schwarz TC, Jeffries CM, Rodriguez Chamorro A, Kostan J, Ghisleni A, Drepper F, Polyansky A, De Almeida Ribeiro E, Pedron M, Zawadzka-Kazimierczuk A, Mlynek G, Peterbauer T, Doto P, Schreiner C, Hollerl E, Mateos B, Geist L, Faulkner G, Kozminski W, Svergun DI, Warscheid B, Zagrovic B, Gautel M, Konrat R, Djinović-Carugo K
|
| RgGuinier |
5.8 |
nm |
| Dmax |
22.5 |
nm |
| VolumePorod |
242 |
nm3 |
|
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