SASBDB entries for UniProt ID: P61981

SASDSJ7 – Human C-terminally truncated 14-3-3 gamma

UniProt ID: P61981 (1-234) 14-3-3 protein gamma
14-3-3 protein gamma experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: 14-3-3 protein gamma dimer, 54 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP, 3% (w/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Oct 3
14-3-3 protein inhibits CaMKK1 by blocking the kinase active site with its last two C-terminal helices. Protein Sci :e4805 (2023)
Petrvalska O, Honzejkova K, Koupilova N, Herman P, Obsilova V, Obsil T
RgGuinier 2.8 nm
Dmax 8.4 nm
VolumePorod 76 nm3

SASDS46 – Human phosphorylated calcium/calmodulin-dependent protein kinase kinase 1 (CaMKK1) bound to 14-3-3 protein gamma

UniProt ID: Q8N5S9 (67-481) Calcium/calmodulin-dependent protein kinase kinase 1 (M474L)

UniProt ID: P61981 (1-234) 14-3-3 protein gamma
Calcium/calmodulin-dependent protein kinase kinase 1 (M474L)14-3-3 protein gamma experimental SAS data
CORAL model
Sample: Calcium/calmodulin-dependent protein kinase kinase 1 (M474L) monomer, 46 kDa Homo sapiens protein
14-3-3 protein gamma dimer, 54 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP, 3% (w/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Oct 23
14-3-3 protein inhibits CaMKK1 by blocking the kinase active site with its last two C-terminal helices. Protein Sci :e4805 (2023)
Petrvalska O, Honzejkova K, Koupilova N, Herman P, Obsilova V, Obsil T
RgGuinier 3.3 nm
Dmax 10.8 nm
VolumePorod 152 nm3

SASDSX7 – Human phosphorylated CaMKK2 bound to 14-3-3 gamma

UniProt ID: P61981 (1-234) 14-3-3 protein gamma

UniProt ID: Q96RR4 (93-517) Calcium/calmodulin-dependent protein kinase kinase 2
14-3-3 protein gammaCalcium/calmodulin-dependent protein kinase kinase 2 experimental SAS data
ALLOSMOD model
Sample: 14-3-3 protein gamma dimer, 54 kDa Homo sapiens protein
Calcium/calmodulin-dependent protein kinase kinase 2 monomer, 48 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP, 3% (w/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Oct 23
14-3-3 protein inhibits CaMKK1 by blocking the kinase active site with its last two C-terminal helices. Protein Sci :e4805 (2023)
Petrvalska O, Honzejkova K, Koupilova N, Herman P, Obsilova V, Obsil T
RgGuinier 3.7 nm
Dmax 13.2 nm
VolumePorod 157 nm3