SASBDB entries for UniProt ID: P70188

SASDMU5 – Kinesin superfamily protein 3 B/B and kinesin associated protein 3 (KAP)

UniProt ID: P70188 (1-693) Kinesin-associated protein 3

UniProt ID: Q61771 (475-747) Kinesin-like protein KIF3B
Kinesin-associated protein 3Kinesin-like protein KIF3B experimental SAS data
DAMMIF model
Sample: Kinesin-associated protein 3 monomer, 81 kDa Mus musculus protein
Kinesin-like protein KIF3B dimer, 64 kDa Mus musculus protein
Buffer: 20 mM Tris-HCl, 200 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Feb 14
Kinesin protein 3 B/B and kinesin associated protein 3 (KAP)
Kento Yonezawa
RgGuinier 5.7 nm
Dmax 28.0 nm
VolumePorod 385 nm3

SASDMV5 – Kinesin superfamily protein 3 A/B and kinesin associated protein 3 (KAP)

UniProt ID: Q61771 (475-747) Kinesin-like protein KIF3B

UniProt ID: P70188 (1-693) Kinesin-associated protein 3

UniProt ID: P28741 (481-701) Kinesin-like protein KIF3A
Kinesin-like protein KIF3BKinesin-associated protein 3Kinesin-like protein KIF3A experimental SAS data
DAMMIF model
Sample: Kinesin-like protein KIF3B monomer, 32 kDa Mus musculus protein
Kinesin-associated protein 3 monomer, 81 kDa Mus musculus protein
Kinesin-like protein KIF3A monomer, 28 kDa Mus musculus protein
Buffer: 20 mM Tris-HCl, 200 mM NaCl, 5% glycerol, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Jun 24
The two-step cargo recognition mechanism of heterotrimeric kinesin. EMBO Rep :e56864 (2023)
Jiang X, Ogawa T, Yonezawa K, Shimizu N, Ichinose S, Uchihashi T, Nagaike W, Moriya T, Adachi N, Kawasaki M, Dohmae N, Senda T, Hirokawa N
RgGuinier 5.8 nm
Dmax 27.0 nm
VolumePorod 557 nm3

SASDMW5 – Kinesin superfamily protein 3 A/B and kinesin associated protein 3 (KAP) with APC ARM

UniProt ID: Q61315 (338-1010) Adenomatous polyposis coli protein (N-terminal ARM domain)

UniProt ID: P28741 (481-701) Kinesin-like protein KIF3A

UniProt ID: Q61771 (475-747) Kinesin-like protein KIF3B

UniProt ID: P70188 (1-692) Kinesin-associated protein 3
Adenomatous polyposis coli protein (N-terminal ARM domain)Kinesin-like protein KIF3AKinesin-like protein KIF3BKinesin-associated protein 3 experimental SAS data
DAMMIF model
Sample: Adenomatous polyposis coli protein (N-terminal ARM domain) monomer, 75 kDa Mus musculus protein
Kinesin-like protein KIF3A monomer, 28 kDa Mus musculus protein
Kinesin-like protein KIF3B monomer, 32 kDa Mus musculus protein
Kinesin-associated protein 3 monomer, 81 kDa Mus musculus protein
Buffer: 25 mM Tris-HCl, 200 mM NaCl, 1 mM DTT, 5% glycerol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Nov 17
The two-step cargo recognition mechanism of heterotrimeric kinesin. EMBO Rep :e56864 (2023)
Jiang X, Ogawa T, Yonezawa K, Shimizu N, Ichinose S, Uchihashi T, Nagaike W, Moriya T, Adachi N, Kawasaki M, Dohmae N, Senda T, Hirokawa N
RgGuinier 5.4 nm
Dmax 19.5 nm
VolumePorod 763 nm3