SASBDB entries for UniProt ID: P84233

SASDFK7 – Complex with 1H histone chaperone Asf1, histones H3 (35-135aa) and H4; 70%-2H histone acetyltransferase Rtt109 and histone chaperone Vps75 (1-225aa) acquired in 100% v/v D2O

UniProt ID: P53853 (1-225) Vacuolar protein sorting-associated protein 75 (1-225 aa)

UniProt ID: Q07794 (1-436) Histone acetyltransferase RTT109

UniProt ID: P32447 (1-169) Histone chaperone ASF1

UniProt ID: P84233 (35-135) Histone H3.2 (35-135 aa)

UniProt ID: P62799 (1-103) Histone H4
Vacuolar protein sorting-associated protein 75 (1-225 aa)Histone acetyltransferase RTT109Histone chaperone ASF1Histone H3.2 (35-135 aa)Histone H4 experimental SAS data
HADDOCK model
Sample: Vacuolar protein sorting-associated protein 75 (1-225 aa) dimer, 53 kDa Saccharomyces cerevisiae protein
Histone acetyltransferase RTT109 monomer, 50 kDa Saccharomyces cerevisiae protein
Histone chaperone ASF1 monomer, 19 kDa protein
Histone H3.2 (35-135 aa) monomer, 12 kDa Xenopus laevis protein
Histone H4 monomer, 11 kDa Xenopus laevis protein
Buffer: 50 mM citrate, 150 mM NaCl, 5 mM BME, 100% D2O, pH: 6.5
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2016 Nov 14
Histone chaperone exploits intrinsic disorder to switch acetylation specificity (Asf1-H3:H4-Rtt109-Vps75 protein complex, data for docking block selections) Nat Commun 10(1):3435 (2019)
Danilenko N, Lercher L, Kirkpatrick J, Gabel F, Codutti L, Carlomagno T
RgGuinier 1.9 nm
Dmax 5.5 nm

SASDSX5 – Nucleosome core particle (NCP) composed of the Widom 601 DNA

UniProt ID: P84233 (1-135) Histone H3.2

UniProt ID: P62799 (1-103) Histone H4

UniProt ID: Q6AZJ8 (1-130) Histone H2A

UniProt ID: P02281 (1-126) Histone H2B 1.1

UniProt ID: None (None-None) Widom 601 DNA sequence
Histone H3.2Histone H4Histone H2AHistone H2B 1.1Widom 601 DNA sequence experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Histone H3.2 dimer, 31 kDa Xenopus laevis protein
Histone H4 dimer, 23 kDa Xenopus laevis protein
Histone H2A dimer, 28 kDa Xenopus laevis protein
Histone H2B 1.1 dimer, 28 kDa Xenopus laevis protein
Widom 601 DNA sequence dimer, 89 kDa Escherichia coli DNA
Buffer: 20 mM Tris-HCl , 50 mM KCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Dec 2
BCL7 proteins, novel subunits of the mammalian SWI/SNF complex, bind the nucleosome core particle
Asgar Abbas kazrani
RgGuinier 4.3 nm
Dmax 12.3 nm
VolumePorod 350 nm3

SASDSY5 – Nucleosome (NCP) in complex with B cell lymphoma 7 protein family member A (BCL7A)

UniProt ID: Q4VC05-2 (1-231) Isoform 2 of B-cell CLL/lymphoma 7 protein family member A

UniProt ID: P84233 (1-135) Histone H3.2

UniProt ID: P62799 (1-103) Histone H4

UniProt ID: Q6AZJ8 (1-130) Histone H2A

UniProt ID: P02281 (1-126) Histone H2B 1.1

UniProt ID: None (None-None) Widom 601 DNA sequence
Isoform 2 of B-cell CLL/lymphoma 7 protein family member AHistone H3.2Histone H4Histone H2AHistone H2B 1.1Widom 601 DNA sequence experimental SAS data
EOM/RANCH model
Sample: Isoform 2 of B-cell CLL/lymphoma 7 protein family member A monomer, 25 kDa Homo sapiens protein
Histone H3.2 dimer, 31 kDa Xenopus laevis protein
Histone H4 dimer, 23 kDa Xenopus laevis protein
Histone H2A dimer, 28 kDa Xenopus laevis protein
Histone H2B 1.1 dimer, 28 kDa Xenopus laevis protein
Widom 601 DNA sequence dimer, 89 kDa Escherichia coli DNA
Buffer: 20 mM Tris-HCl , 50 mM KCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Dec 2
BCL7 proteins, novel subunits of the mammalian SWI/SNF complex, bind the nucleosome core particle
Asgar Abbas kazrani
RgGuinier 4.7 nm
Dmax 24.4 nm
VolumePorod 400 nm3