Small Angle Scattering Biological Data Bank SASBDB

SASBDB entries for UniProt ID: P9WGY7

SASDJK4 – RNA polymerase core complex

UniProt ID: P9WGZ1 (1-347) DNA-directed RNA polymerase subunit alpha

UniProt ID: P9WGY9 (7-1178) DNA-directed RNA polymerase subunit beta

UniProt ID: P9WGY7 (2-1316) DNA-directed RNA polymerase subunit beta'

UniProt ID: P9WGY5 (2-110) DNA-directed RNA polymerase subunit omega

DNA-directed RNA polymerase subunit alphaDNA-directed RNA polymerase subunit betaDNA-directed RNA polymerase subunit beta'DNA-directed RNA polymerase subunit omega experimental SAS data

Sample:	DNA-directed RNA polymerase subunit alpha dimer, 78 kDa Mycobacterium tuberculosis protein DNA-directed RNA polymerase subunit beta monomer, 129 kDa Mycobacterium tuberculosis protein DNA-directed RNA polymerase subunit beta' monomer, 147 kDa Mycobacterium tuberculosis protein DNA-directed RNA polymerase subunit omega monomer, 12 kDa Mycobacterium tuberculosis protein
Buffer:	10 mM Tris-Cl, 100 mM NaCl, 10 mM MgCl2, 0.1 mM EDTA, 5% Glycerol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2018 Mar 8

Structural and biochemical characterization of RNA polymerase core and UvrD complex: a key component in transcription coupled DNA repair
Ravishankar Ramachandran

R_g^Guinier	5.1	nm
D_max	19.3	nm
Volume^Porod	262	nm³

SASDJL4 – RNA polymerase core subunit in complex with ATP-dependent DNA helicase UvrD1

UniProt ID: P9WGZ1 (1-347) DNA-directed RNA polymerase subunit alpha

UniProt ID: P9WGY9 (7-1178) DNA-directed RNA polymerase subunit beta

UniProt ID: P9WGY7 (2-1316) DNA-directed RNA polymerase subunit beta'

UniProt ID: P9WGY5 (1-110) DNA-directed RNA polymerase subunit omega

UniProt ID: P9WMQ1 (1-771) ATP-dependent DNA helicase UvrD1

Sample:	DNA-directed RNA polymerase subunit alpha dimer, 75 kDa Mycobacterium tuberculosis protein DNA-directed RNA polymerase subunit beta monomer, 129 kDa Mycobacterium tuberculosis protein DNA-directed RNA polymerase subunit beta' monomer, 147 kDa Mycobacterium tuberculosis protein DNA-directed RNA polymerase subunit omega monomer, 12 kDa Mycobacterium tuberculosis protein ATP-dependent DNA helicase UvrD1 monomer, 85 kDa Mycobacterium tuberculosis protein
Buffer:	10 mM Tris-Cl, 100 mM NaCl, 10 mM MgCl2, 0.1 mM EDTA, 5% Glycerol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2018 Mar 11

Structural and biochemical characterization of RNA polymerase core and UvrD complex: a key component in transcription coupled DNA repair
Ravishankar Ramachandran

R_g^Guinier	7.5	nm
D_max	22.2	nm
Volume^Porod	898	nm³