SASBDB entries for UniProt ID: Q00623

SASDC99 – Nanolipoprotein Particle (NLP) from In Vitro Assembly - 100% D2O SANS

UniProt ID: Q00623 (67-264) Apolipoprotein A-I

UniProt ID: None (None-None) 1,2-dimyristoyl-sn-glycero-3-phosphocholine

Apolipoprotein A-I1,2-dimyristoyl-sn-glycero-3-phosphocholine experimental SAS data
Apolipoprotein A-I 1,2-dimyristoyl-sn-glycero-3-phosphocholine Kratky plot
Sample: Apolipoprotein A-I dimer, 50 kDa Mus musculus protein
1,2-dimyristoyl-sn-glycero-3-phosphocholine, 92 kDa
Buffer: PBS in D2O, pH: 7.4
Experiment: SANS data collected at NG7, NIST Center for Neutron Research on 2015 Nov 25
Small-angle X-ray and neutron scattering demonstrates that cell-free expression produces properly formed disc-shaped nanolipoprotein particles. Protein Sci 27(3):780-789 (2018)
Cleveland TE 4th, He W, Evans AC, Fischer NO, Lau EY, Coleman MA, Butler P
RgGuinier 3.1 nm
Dmax 9.1 nm

SASDCA9 – Nanolipoprotein Particle (NLP) from In Vitro Assembly - SAXS

UniProt ID: Q00623 (67-264) Apolipoprotein A-I

UniProt ID: None (None-None) 1,2-dimyristoyl-sn-glycero-3-phosphocholine

Apolipoprotein A-I1,2-dimyristoyl-sn-glycero-3-phosphocholine experimental SAS data
Apolipoprotein A-I 1,2-dimyristoyl-sn-glycero-3-phosphocholine Kratky plot
Sample: Apolipoprotein A-I dimer, 50 kDa Mus musculus protein
1,2-dimyristoyl-sn-glycero-3-phosphocholine, 92 kDa
Buffer: PBS in D2O, pH: 7.4
Experiment: SAXS data collected at SAXSLAB Bio-Ganesha, NIST/IBBR on 2015 Nov 24
Small-angle X-ray and neutron scattering demonstrates that cell-free expression produces properly formed disc-shaped nanolipoprotein particles. Protein Sci 27(3):780-789 (2018)
Cleveland TE 4th, He W, Evans AC, Fischer NO, Lau EY, Coleman MA, Butler P
RgGuinier 4.8 nm
Dmax 12.0 nm

SASDCB9 – Nanolipoprotein Particle (NLP) assembled with tail-deuterated DMPC - 100% D2O SANS

UniProt ID: Q00623 (67-264) Apolipoprotein A-I

UniProt ID: None (None-None) 1,2-dimyristoyl-d54-sn-glycero-3-phosphocholine

Apolipoprotein A-I1,2-dimyristoyl-d54-sn-glycero-3-phosphocholine experimental SAS data
Apolipoprotein A-I 1,2-dimyristoyl-d54-sn-glycero-3-phosphocholine Kratky plot
Sample: Apolipoprotein A-I dimer, 50 kDa Mus musculus protein
1,2-dimyristoyl-d54-sn-glycero-3-phosphocholine, 99 kDa
Buffer: PBS in D2O, pH: 7.4
Experiment: SANS data collected at NG7, NIST Center for Neutron Research on 2016 Apr 11
Small-angle X-ray and neutron scattering demonstrates that cell-free expression produces properly formed disc-shaped nanolipoprotein particles. Protein Sci 27(3):780-789 (2018)
Cleveland TE 4th, He W, Evans AC, Fischer NO, Lau EY, Coleman MA, Butler P
RgGuinier 4.3 nm
Dmax 10.7 nm

SASDCC9 – Nanolipoprotein Particle (NLP) from In Vitro Assembly - 42% D2O SANS

UniProt ID: Q00623 (67-264) Apolipoprotein A-I

UniProt ID: None (None-None) 1,2-dimyristoyl-sn-glycero-3-phosphocholine

Apolipoprotein A-I1,2-dimyristoyl-sn-glycero-3-phosphocholine experimental SAS data
Apolipoprotein A-I 1,2-dimyristoyl-sn-glycero-3-phosphocholine Kratky plot
Sample: Apolipoprotein A-I dimer, 50 kDa Mus musculus protein
1,2-dimyristoyl-sn-glycero-3-phosphocholine, 92 kDa
Buffer: PBS in 42% D2O, pH: 7.4
Experiment: SANS data collected at NGB30, NIST Center for Neutron Research on 2015 Dec 11
Small-angle X-ray and neutron scattering demonstrates that cell-free expression produces properly formed disc-shaped nanolipoprotein particles. Protein Sci 27(3):780-789 (2018)
Cleveland TE 4th, He W, Evans AC, Fischer NO, Lau EY, Coleman MA, Butler P
RgGuinier 2.9 nm
Dmax 7.3 nm

SASDCD9 – Nanolipoprotein Particle (NLP) from Cell-Free Expression - SAXS

UniProt ID: None (None-None) 1,2-dimyristoyl-sn-glycero-3-phosphocholine

UniProt ID: Q00623 (67-264) Apolipoprotein A-I

1,2-dimyristoyl-sn-glycero-3-phosphocholineApolipoprotein A-I experimental SAS data
1,2-dimyristoyl-sn-glycero-3-phosphocholine Apolipoprotein A-I Kratky plot
Sample: 1,2-dimyristoyl-sn-glycero-3-phosphocholine, 92 kDa
Apolipoprotein A-I dimer, 52 kDa Mus musculus protein
Buffer: PBS in D2O, pH: 7.4
Experiment: SAXS data collected at SAXSLAB Bio-Ganesha, NIST/IBBR on 2015 Nov 24
Small-angle X-ray and neutron scattering demonstrates that cell-free expression produces properly formed disc-shaped nanolipoprotein particles. Protein Sci 27(3):780-789 (2018)
Cleveland TE 4th, He W, Evans AC, Fischer NO, Lau EY, Coleman MA, Butler P
RgGuinier 4.7 nm
Dmax 12.7 nm

SASDCE9 – Nanolipoprotein Particle (NLP) with Telodendrimer - SAXS

UniProt ID: None (None-None) 1,2-dimyristoyl-sn-glycero-3-phosphocholine

UniProt ID: Q00623 (67-264) Apolipoprotein A-I

1,2-dimyristoyl-sn-glycero-3-phosphocholineApolipoprotein A-I experimental SAS data
1,2-dimyristoyl-sn-glycero-3-phosphocholine Apolipoprotein A-I Kratky plot
Sample: 1,2-dimyristoyl-sn-glycero-3-phosphocholine, 92 kDa
Apolipoprotein A-I dimer, 52 kDa Mus musculus protein
Buffer: PBS in D2O, pH: 7.4
Experiment: SAXS data collected at SAXSLAB Bio-Ganesha, NIST/IBBR on 2015 Nov 24
Small-angle X-ray and neutron scattering demonstrates that cell-free expression produces properly formed disc-shaped nanolipoprotein particles. Protein Sci 27(3):780-789 (2018)
Cleveland TE 4th, He W, Evans AC, Fischer NO, Lau EY, Coleman MA, Butler P
RgGuinier 4.4 nm
Dmax 12.7 nm

SASDCF9 – Nanolipoprotein Particle (NLP) from Cell-Free Expression - 100% D2O SANS

UniProt ID: None (None-None) 1,2-dimyristoyl-sn-glycero-3-phosphocholine

UniProt ID: Q00623 (67-264) Apolipoprotein A-I

1,2-dimyristoyl-sn-glycero-3-phosphocholineApolipoprotein A-I experimental SAS data
1,2-dimyristoyl-sn-glycero-3-phosphocholine Apolipoprotein A-I Kratky plot
Sample: 1,2-dimyristoyl-sn-glycero-3-phosphocholine, 92 kDa
Apolipoprotein A-I dimer, 52 kDa Mus musculus protein
Buffer: PBS in D2O, pH: 7.4
Experiment: SANS data collected at NG7, NIST Center for Neutron Research on 2015 Nov 25
Small-angle X-ray and neutron scattering demonstrates that cell-free expression produces properly formed disc-shaped nanolipoprotein particles. Protein Sci 27(3):780-789 (2018)
Cleveland TE 4th, He W, Evans AC, Fischer NO, Lau EY, Coleman MA, Butler P
RgGuinier 3.5 nm
Dmax 12.5 nm

SASDCG9 – Nanolipoprotein Particle (NLP) with Telodendrimer - 100% D2O SANS

UniProt ID: None (None-None) 1,2-dimyristoyl-sn-glycero-3-phosphocholine

UniProt ID: Q00623 (67-264) Apolipoprotein A-I

1,2-dimyristoyl-sn-glycero-3-phosphocholineApolipoprotein A-I experimental SAS data
1,2-dimyristoyl-sn-glycero-3-phosphocholine Apolipoprotein A-I Kratky plot
Sample: 1,2-dimyristoyl-sn-glycero-3-phosphocholine, 92 kDa
Apolipoprotein A-I dimer, 52 kDa Mus musculus protein
Buffer: PBS in D2O, pH: 7.4
Experiment: SANS data collected at NG7, NIST Center for Neutron Research on 2015 Nov 25
Small-angle X-ray and neutron scattering demonstrates that cell-free expression produces properly formed disc-shaped nanolipoprotein particles. Protein Sci 27(3):780-789 (2018)
Cleveland TE 4th, He W, Evans AC, Fischer NO, Lau EY, Coleman MA, Butler P
RgGuinier 3.3 nm
Dmax 10.0 nm