SASBDB entries for UniProt ID: Q4VGL6

SASDNW5 – apo Roquin-1 (coreROQ) at 50 µM, including concentration series data at 100, 200, 300 µM

UniProt ID: Q4VGL6 (171-326) Roquin-1
Roquin-1 experimental SAS data
Roquin-1 Kratky plot
Sample: Roquin-1 monomer, 18 kDa Mus musculus protein
Buffer: 150 mM NaCl, 20 mM Tris, 2 mM TCEP, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 1
NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
Tants JN, Becker LM, McNicoll F, Müller-McNicoll M, Schlundt A
RgGuinier 1.9 nm
Dmax 7.0 nm
VolumePorod 35 nm3

SASDNX5 – apo Roquin-1 (extROQ) at 50 µM, including concentration series data at 100, 200, 300 µM

UniProt ID: Q4VGL6 (89-404) Roquin-1 extROQ
Roquin-1 extROQ experimental SAS data
Roquin-1 extROQ Kratky plot
Sample: Roquin-1 extROQ monomer, 35 kDa Mus musculus protein
Buffer: 150 mM NaCl, 20 mM Tris, 2 mM TCEP, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 1
NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
Tants JN, Becker LM, McNicoll F, Müller-McNicoll M, Schlundt A
RgGuinier 2.8 nm
Dmax 14.0 nm
VolumePorod 53 nm3

SASDNY5 – apo Roquin-1 (Nterm ROQ) at 50 µM, including concentration series data at 100, 300 µM

UniProt ID: Q4VGL6 (1-454) Roquin-1 Nterm
Roquin-1 Nterm experimental SAS data
Roquin-1 Nterm Kratky plot
Sample: Roquin-1 Nterm monomer, 51 kDa Mus musculus protein
Buffer: 150 mM NaCl, 20 mM Tris, 2 mM TCEP, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 1
NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
Tants JN, Becker LM, McNicoll F, Müller-McNicoll M, Schlundt A
RgGuinier 3.4 nm
Dmax 15.0 nm
VolumePorod 88 nm3

SASDNZ5 – apo Roquin-1 (Nterm A-site mut ROQ) at 50 µM, including concentration series data at 100, 300 µM

UniProt ID: Q4VGL6 (1-454) Roquin-1 Nterm Amut (K220A, K239A, R260A)
Roquin-1 Nterm Amut (K220A, K239A, R260A) experimental SAS data
Roquin-1 Nterm Amut (K220A, K239A, R260A) Kratky plot
Sample: Roquin-1 Nterm Amut (K220A, K239A, R260A) monomer, 51 kDa Mus musculus protein
Buffer: 150 mM NaCl, 20 mM Tris, 2 mM TCEP, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 1
NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
Tants JN, Becker LM, McNicoll F, Müller-McNicoll M, Schlundt A
RgGuinier 3.3 nm
Dmax 14.0 nm
VolumePorod 80 nm3

SASDN26 – apo Roquin-1 (Nterm B-site mut ROQ) at 50 µM, including concentration series data at 100, 300 µM

UniProt ID: Q4VGL6 (1-454) Roquin-1 Nterm Bmut (R135E, K136E, D322A, K323A)
Roquin-1 Nterm Bmut (R135E, K136E, D322A, K323A) experimental SAS data
Roquin-1 Nterm Bmut (R135E, K136E, D322A, K323A) Kratky plot
Sample: Roquin-1 Nterm Bmut (R135E, K136E, D322A, K323A) monomer, 51 kDa Mus musculus protein
Buffer: 150 mM NaCl, 20 mM Tris, 2 mM TCEP, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 1
NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
Tants JN, Becker LM, McNicoll F, Müller-McNicoll M, Schlundt A
RgGuinier 3.5 nm
Dmax 15.0 nm
VolumePorod 92 nm3