SASBDB entries for UniProt ID: Q86U28

SASDGE2 – Human mitochondrial iron-sulfur cluster assembly ISCA2 protein

UniProt ID: Q86U28 (44-154) Iron-sulfur cluster assembly 2 homolog, mitochondrial
Iron-sulfur cluster assembly 2 homolog, mitochondrial experimental SAS data
SASREF model
Sample: Iron-sulfur cluster assembly 2 homolog, mitochondrial dimer, 24 kDa Homo sapiens protein
Buffer: 50 mM phosphate, 150 mM NaCl, 5 mM DTT, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Sep 16
Structural properties of [2Fe-2S] ISCA2-IBA57: a complex of the mitochondrial iron-sulfur cluster assembly machinery Scientific Reports 9(1) (2019)
Nasta V, Da Vela S, Gourdoupis S, Ciofi-Baffoni S, Svergun D, Banci L
RgGuinier 2.0 nm
Dmax 6.7 nm
VolumePorod 31 nm3

SASDGF2 – Putative mitochondrial transferase CAF17 bound to mitochondrial iron-sulfur cluster assembly ISCA2 protein (human IBA57-ISCA2 complex)

UniProt ID: Q5T440 (44-352) Putative transferase CAF17, mitochondrial

UniProt ID: Q86U28 (44-154) Iron-sulfur cluster assembly 2 homolog, mitochondrial
Putative transferase CAF17, mitochondrialIron-sulfur cluster assembly 2 homolog, mitochondrial experimental SAS data
SASREF CV model
Sample: Putative transferase CAF17, mitochondrial monomer, 33 kDa Homo sapiens protein
Iron-sulfur cluster assembly 2 homolog, mitochondrial dimer, 24 kDa Homo sapiens protein
Buffer: 50 mM phosphate, 150 mM NaCl, 5 mM DTT, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Nov 22
Structural properties of [2Fe-2S] ISCA2-IBA57: a complex of the mitochondrial iron-sulfur cluster assembly machinery Scientific Reports 9(1) (2019)
Nasta V, Da Vela S, Gourdoupis S, Ciofi-Baffoni S, Svergun D, Banci L
RgGuinier 4.0 nm
Dmax 16.4 nm
VolumePorod 114 nm3

SASDPC8 – Human apo iron-sulfur cluster assembly 1/iron-sulfur cluster assembly 2 complex (ISCA1-ISCA2)

UniProt ID: Q9BUE6 (1-129) Iron-sulfur cluster assembly 1 homolog, mitochondrial

UniProt ID: Q86U28 (44-154) Iron-sulfur cluster assembly 2 homolog, mitochondrial
Iron-sulfur cluster assembly 1 homolog, mitochondrialIron-sulfur cluster assembly 2 homolog, mitochondrial experimental SAS data
DAMMIF model
Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial monomer, 14 kDa Homo sapiens protein
Iron-sulfur cluster assembly 2 homolog, mitochondrial monomer, 13 kDa Homo sapiens protein
Buffer: 50 mM phosphate, 150 mM NaCl, 5 mM DTT, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 27
Structural plasticity of NFU1 upon interaction with binding partners: insights into the mitochondrial [4Fe-4S] cluster pathway Journal of Molecular Biology :168154 (2023)
Da Vela S, Saudino G, Lucarelli F, Banci L, Svergun D, Ciofi-Baffoni S
RgGuinier 2.4 nm
Dmax 7.8 nm
VolumePorod 43 nm3

SASDPD8 – Human apo iron-sulfur cluster assembly 1/iron-sulfur cluster assembly 2/NFU1 iron-sulfur cluster scaffold protein complex (ISCA1-ISCA2-NFU1)

UniProt ID: Q9BUE6 (1-129) Iron-sulfur cluster assembly 1 homolog, mitochondrial

UniProt ID: Q9UMS0 (59-254) NFU1 iron-sulfur cluster scaffold homolog, mitochondrial (F118S, E168G)

UniProt ID: Q86U28 (44-154) Iron-sulfur cluster assembly 2 homolog, mitochondrial
Iron-sulfur cluster assembly 1 homolog, mitochondrialNFU1 iron-sulfur cluster scaffold homolog, mitochondrial (F118S, E168G)Iron-sulfur cluster assembly 2 homolog, mitochondrial experimental SAS data
CORAL model
Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial monomer, 14 kDa Homo sapiens protein
NFU1 iron-sulfur cluster scaffold homolog, mitochondrial (F118S, E168G) monomer, 22 kDa Homo sapiens protein
Iron-sulfur cluster assembly 2 homolog, mitochondrial monomer, 13 kDa Homo sapiens protein
Buffer: 50 mM phosphate, 150 mM NaCl, 5 mM DTT, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 27
Structural plasticity of NFU1 upon interaction with binding partners: insights into the mitochondrial [4Fe-4S] cluster pathway Journal of Molecular Biology :168154 (2023)
Da Vela S, Saudino G, Lucarelli F, Banci L, Svergun D, Ciofi-Baffoni S
RgGuinier 3.2 nm
Dmax 11.7 nm
VolumePorod 70 nm3