SASBDB entries for UniProt ID: Q8WZ42

SASDKA2 – Two titin I27 domains linked with RS linker

UniProt ID: Q8WZ42 (None-None) Two titin I27 domains linked with RS linker
Two titin I27 domains linked with RS linker experimental SAS data
Two titin I27 domains linked with RS linker Rg histogram
Sample: Two titin I27 domains linked with RS linker monomer, 23 kDa protein
Buffer: 50 mM potassium phosphate, 300 mM KCl, DTT 2mM, pH: 7.6
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Dec 4
Interdomain linkers tailor the stability of immunoglobulin repeats in polyproteins. Biochem Biophys Res Commun 550:43-48 (2021)
Joshi T, Garg S, Estaña A, Cortés J, Bernadó P, Das S, Kammath AR, Sagar A, Rakshit S
RgGuinier 3.0 nm

SASDKB2 – Two titin I27 domains linked with RS linker

UniProt ID: Q8WZ42 (None-None) Two titin I27 domains linked with RS linker
Two titin I27 domains linked with RS linker experimental SAS data
Sample: Two titin I27 domains linked with RS linker monomer, 23 kDa protein
Buffer: 50 mM potassium phosphate, 300 mM KCl, DTT 2mM, pH: 7.6
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Dec 4
Interdomain linkers tailor the stability of immunoglobulin repeats in polyproteins. Biochem Biophys Res Commun 550:43-48 (2021)
Joshi T, Garg S, Estaña A, Cortés J, Bernadó P, Das S, Kammath AR, Sagar A, Rakshit S
RgGuinier 3.0 nm

SASDKC2 – Two titin I27 domains linked with GGG linker

UniProt ID: Q8WZ42 (None-None) Two titin I27 domains linked with GGG linker
Two titin I27 domains linked with GGG linker experimental SAS data
Two titin I27 domains linked with GGG linker Rg histogram
Sample: Two titin I27 domains linked with GGG linker monomer, 23 kDa protein
Buffer: 50 mM potassium phosphate, 300 mM KCl, DTT 2mM, pH: 7.6
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Dec 4
Interdomain linkers tailor the stability of immunoglobulin repeats in polyproteins. Biochem Biophys Res Commun 550:43-48 (2021)
Joshi T, Garg S, Estaña A, Cortés J, Bernadó P, Das S, Kammath AR, Sagar A, Rakshit S
RgGuinier 2.9 nm

SASDKD2 – Two titin I27 domains linked with VKA linker

UniProt ID: Q8WZ42 (None-None) Two titin I27 domains linked with VKA linker
Two titin I27 domains linked with VKA linker experimental SAS data
Two titin I27 domains linked with VKA linker Rg histogram
Sample: Two titin I27 domains linked with VKA linker monomer, 24 kDa protein
Buffer: 50 mM potassium phosphate, 300 mM KCl, DTT 2mM, pH: 7.6
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Dec 4
Interdomain linkers tailor the stability of immunoglobulin repeats in polyproteins. Biochem Biophys Res Commun 550:43-48 (2021)
Joshi T, Garg S, Estaña A, Cortés J, Bernadó P, Das S, Kammath AR, Sagar A, Rakshit S
RgGuinier 3.1 nm

SASDKE2 – Two titin I27 domains linked with AAE linker

UniProt ID: Q8WZ42 (None-None) Two titin I27 domains linked with AAE linker
Two titin I27 domains linked with AAE linker experimental SAS data
Two titin I27 domains linked with AAE linker Rg histogram
Sample: Two titin I27 domains linked with AAE linker monomer, 24 kDa protein
Buffer: 50 mM potassium phosphate, 300 mM KCl, DTT 2mM, pH: 7.6
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Dec 4
Interdomain linkers tailor the stability of immunoglobulin repeats in polyproteins. Biochem Biophys Res Commun 550:43-48 (2021)
Joshi T, Garg S, Estaña A, Cortés J, Bernadó P, Das S, Kammath AR, Sagar A, Rakshit S
RgGuinier 3.1 nm

SASDMN9 – Conformational characterization of A77-78 fibronectin type III tandem

UniProt ID: Q8WZ42 (None-None) Titin
Titin experimental SAS data
OTHER model
Sample: Titin monomer, 22 kDa Homo sapiens protein
Buffer: 100 mM NaCl, 50 mM Tris-HCl, 2mM DTT, pH: 7.2
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Jul 3
The Structure of the FnIII Tandem A77-A78 Points to a Periodically Conserved Architecture in the Myosin-Binding Region of Titin Journal of Molecular Biology 401(5):843-853 (2010)
Bucher R, Svergun D, Muhle-Goll C, Mayans O
RgGuinier 2.5 nm
Dmax 90.0 nm
VolumePorod 21 nm3

SASDMP9 – Conformational characterization of A80-82 fibronectin type III tandem

UniProt ID: Q8WZ42 (None-None) Titin
Titin experimental SAS data
SASREF model
Sample: Titin monomer, 32 kDa Homo sapiens protein
Buffer: 100 mM NaCl, 50 mM Tris-HCl, 2mM DTT, pH: 7.2
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Jul 3
The Structure of the FnIII Tandem A77-A78 Points to a Periodically Conserved Architecture in the Myosin-Binding Region of Titin Journal of Molecular Biology 401(5):843-853 (2010)
Bucher R, Svergun D, Muhle-Goll C, Mayans O
RgGuinier 3.7 nm
Dmax 130.0 nm
VolumePorod 39 nm3

SASDMQ9 – Conformational characterization of A84-86 fibronectin type III tandem

UniProt ID: Q8WZ42 (None-None) Titin
Titin experimental SAS data
SASREF model
Sample: Titin monomer, 32 kDa Homo sapiens protein
Buffer: 100 mM NaCl, 50 mM Tris-HCl, 2mM DTT, pH: 7.2
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Oct 6
The Structure of the FnIII Tandem A77-A78 Points to a Periodically Conserved Architecture in the Myosin-Binding Region of Titin Journal of Molecular Biology 401(5):843-853 (2010)
Bucher R, Svergun D, Muhle-Goll C, Mayans O
RgGuinier 3.7 nm
Dmax 14.0 nm