SASBDB entries for UniProt ID: Q9H845

SASDHW4 – Mitochondrial Complex I assembly factor, ACAD9

UniProt ID: Q9H845 (38-621) Complex I assembly factor ACAD9, mitochondrial
Complex I assembly factor ACAD9, mitochondrial experimental SAS data
DAMMIN model
Sample: Complex I assembly factor ACAD9, mitochondrial dimer, 132 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 250 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 28
Assembly of the mitochondrial Complex I assembly complex suggests a regulatory role for deflavination. Angew Chem Int Ed Engl (2020)
Giachin G, Jessop M, Bouverot R, Acajjaoui S, Saidi M, Chretien A, Bacia-Verloop M, Signor L, Mas PJ, Favier A, Borel Meneroud E, Hons M, Hart DJ, Kandiah E, Boeri Erba E, Buisson A, Leonard G, Gutsche I, Soler-Lopez M
RgGuinier 3.6 nm
Dmax 10.2 nm
VolumePorod 217 nm3

SASDHZ4 – The C-terminal domain of ESCIT bound to a dimer of the complex I assembly factor ACAD9

UniProt ID: Q9H845 (38-621) Complex I assembly factor ACAD9, mitochondrial

UniProt ID: Q9BQ95 (249-431) Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial
Complex I assembly factor ACAD9, mitochondrialEvolutionarily conserved signaling intermediate in Toll pathway, mitochondrial experimental SAS data
GASBOR model
Sample: Complex I assembly factor ACAD9, mitochondrial dimer, 132 kDa Homo sapiens protein
Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial tetramer, 88 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 250 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Oct 4
Assembly of the mitochondrial Complex I assembly complex suggests a regulatory role for deflavination. Angew Chem Int Ed Engl (2020)
Giachin G, Jessop M, Bouverot R, Acajjaoui S, Saidi M, Chretien A, Bacia-Verloop M, Signor L, Mas PJ, Favier A, Borel Meneroud E, Hons M, Hart DJ, Kandiah E, Boeri Erba E, Buisson A, Leonard G, Gutsche I, Soler-Lopez M
RgGuinier 6.0 nm
Dmax 16.5 nm
VolumePorod 683 nm3

SASDJ34 – Human Complex I assembly factor ACAD9 dimer

UniProt ID: Q9H845 (38-621) Complex I assembly factor ACAD9, mitochondrial
Complex I assembly factor ACAD9, mitochondrial experimental SAS data
CUSTOM IN-HOUSE model
Sample: Complex I assembly factor ACAD9, mitochondrial dimer, 130 kDa Homo sapiens protein
Buffer: 25mM Tris-HCl, 150mM NaCl, 0.1mM DTT, and 5% glycerol, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Nov 2
Molecular mechanism of interactions between ACAD9 and binding partners in mitochondrial respiratory complex I assembly iScience 24(10):103153 (2021)
Xia C, Lou B, Fu Z, Mohsen A, Shen A, Vockley J, Kim J
RgGuinier 3.5 nm
Dmax 13.0 nm
VolumePorod 213 nm3

SASDJ44 – Human Complex I assembly factor ACAD9 complexed with ECSIT

UniProt ID: Q9H845 (38-621) Complex I assembly factor ACAD9, mitochondrial

UniProt ID: Q9BQ95 (49-431) Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial
Complex I assembly factor ACAD9, mitochondrialEvolutionarily conserved signaling intermediate in Toll pathway, mitochondrial experimental SAS data
DAMFILT model
Sample: Complex I assembly factor ACAD9, mitochondrial dimer, 130 kDa Homo sapiens protein
Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial dimer, 93 kDa Homo sapiens protein
Buffer: 25mM Tris-HCl, 150mM NaCl, 0.1mM DTT, and 5% glycerol, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Nov 2
Molecular mechanism of interactions between ACAD9 and binding partners in mitochondrial respiratory complex I assembly iScience 24(10):103153 (2021)
Xia C, Lou B, Fu Z, Mohsen A, Shen A, Vockley J, Kim J
RgGuinier 5.1 nm
Dmax 16.8 nm
VolumePorod 514 nm3

SASDJ54 – Human Complex I assembly factor ACAD9 complexed with ECSIT and SMT3-NDUFAF1

UniProt ID: Q9H845 (38-621) Complex I assembly factor ACAD9, mitochondrial

UniProt ID: Q9BQ95 (49-431) Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial

UniProt ID: Q9Y375 (25-327) Complex I intermediate-associated protein 30, mitochondrial
Complex I assembly factor ACAD9, mitochondrialEvolutionarily conserved signaling intermediate in Toll pathway, mitochondrialComplex I intermediate-associated protein 30, mitochondrial experimental SAS data
DAMMIF model
Sample: Complex I assembly factor ACAD9, mitochondrial tetramer, 259 kDa Homo sapiens protein
Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial tetramer, 184 kDa Homo sapiens protein
Complex I intermediate-associated protein 30, mitochondrial tetramer, 190 kDa Homo sapiens protein
Buffer: 25mM Tris-HCl, 150mM NaCl, 0.1mM DTT, and 5% glycerol, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Nov 2
Molecular mechanism of interactions between ACAD9 and binding partners in mitochondrial respiratory complex I assembly iScience 24(10):103153 (2021)
Xia C, Lou B, Fu Z, Mohsen A, Shen A, Vockley J, Kim J
RgGuinier 6.6 nm
Dmax 21.6 nm
VolumePorod 1340 nm3