Small Angle Scattering Biological Data Bank SASBDB

SASBDB entries for UniProt ID: Q9UBF9

SASDFZ7 – Full-length myotilin with N-terminally fused thioredoxin tag

UniProt ID: Q9UBF9 (2-498) his-trx-myotilin

Full-length myotilin with N-terminally fused thioredoxin tag Rg histogram

Sample:	His-trx-myotilin monomer, 70 kDa Homo sapiens protein
Buffer:	20 mM Tris, 400 mM NaCl, 250 mM arginine, 5% v/v glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Jun 11

Molecular basis of F-actin regulation and sarcomere assembly via myotilin PLOS Biology 19(4):e3001148 (2021)
Kostan J, Pavšič M, Puž V, Schwarz T, Drepper F, Molt S, Graewert M, Schreiner C, Sajko S, van der Ven P, Onipe A, Svergun D, Warscheid B, Konrat R, Fürst D, Lenarčič B, Djinović-Carugo K, Machesky L

R_g^Guinier	5.1	nm
D_max	16.7	nm
Volume^Porod	131	nm³

SASDF28 – Myotilin immunoglobulin domains Ig1Ig2 (220-452)

UniProt ID: Q9UBF9 (220-452) Myotilin Ig1Ig2 (220-452)

Myotilin Ig1Ig2 (220-452) experimental SAS data

Sample:	Myotilin Ig1Ig2 (220-452) monomer, 27 kDa Homo sapiens protein
Buffer:	20 mM Na+-HEPES, 150 mM, NaCl, 5 % v/v glycerol, 1 mM DTT, pH: 7.4
Experiment:	SAXS data collected at BM29, ESRF on 2010 Nov 16

R_g^Guinier	3.7	nm
D_max	15.1	nm
Volume^Porod	52	nm³

SASDF38 – Myotilin immunoglobulin domains Ig1Ig2 (250-444)

UniProt ID: Q9UBF9 (250-444) Myotilin Ig1Ig2 (250-444)

Myotilin Ig1Ig2 (250-444) experimental SAS data

Sample:	Myotilin Ig1Ig2 (250-444) monomer, 22 kDa Homo sapiens protein
Buffer:	20 mM Na+-HEPES, 150 mM, NaCl, 5 % v/v glycerol, 1 mM DTT, pH: 7.4
Experiment:	SAXS data collected at BM29, ESRF on 2019 Jun 27

R_g^Guinier	2.8	nm
D_max	10.1	nm
Volume^Porod	29	nm³

SASDF48 – Myotilin immunoglobulin domains Ig1Ig2 (250-498)

UniProt ID: Q9UBF9 (250-498) Myotilin Ig1Ig2 (250-498)

Myotilin Ig1Ig2 (250-498) experimental SAS data

Sample:	Myotilin Ig1Ig2 (250-498) monomer, 28 kDa Homo sapiens protein
Buffer:	20 mM Na+-HEPES, 150 mM, NaCl, 5 % v/v glycerol, 1 mM DTT, pH: 7.4
Experiment:	SAXS data collected at BM29, ESRF on 2019 Jun 27

R_g^Guinier	3.2	nm
D_max	14.0	nm
Volume^Porod	37	nm³

SASDJH8 – Myotilin immunoglobulin domains Ig1Ig2 (220-452, wild-type) concentration series data

UniProt ID: Q9UBF9 (220-452) Myotilin (222-452)

Myotilin (222-452) experimental SAS data

Sample:	Myotilin (222-452) monomer, 26 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, 5 % glycerol, 1 mM DTT, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Jul 6

R_g^Guinier	3.3	nm
D_max	11.5	nm
Volume^Porod	36	nm³

SASDJJ8 – Myotilin immunoglobulin domains Ig1Ig2 (220-452, R405K mutant) concentration series data

UniProt ID: Q9UBF9 (222-452) Myotilin (222-452) R405K

Myotilin (222-452) R405K experimental SAS data

Sample:	Myotilin (222-452) R405K monomer, 26 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, 5 % glycerol, 1 mM DTT, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Jul 12

R_g^Guinier	3.3	nm
D_max	10.5	nm
Volume^Porod	36	nm³