SASBDB entries for UniProt ID: Q9UBF9

SASDFZ7 – Full-length myotilin with N-terminally fused thioredoxin tag

UniProt ID: Q9UBF9 (2-498) his-trx-myotilin

his-trx-myotilin experimental SAS data
Full-length myotilin with N-terminally fused thioredoxin tag Rg histogram
Sample: His-trx-myotilin monomer, 70 kDa Homo sapiens protein
Buffer: 20 mM Tris, 400 mM NaCl, 250 mM arginine, 5% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jun 11
Structural insights into F-actin regulation and sarcomere assembly via myotilin (2020)
Kostan J, Pavšič M, Puž V, Schwarz T, Drepper F, Molt S, Graewert M, Schreiner C, Sajko S, van der Ven P, Svergun D, Warscheid B, Konrat R, Fürst D, Lenarčič B, Djinović-Carugo K
RgGuinier 5.1 nm
Dmax 16.7 nm
VolumePorod 131 nm3

SASDF28 – Myotilin immunoglobulin domains Ig1Ig2 (220-452)

UniProt ID: Q9UBF9 (220-452) Myotilin Ig1Ig2 (220-452)

Myotilin Ig1Ig2 (220-452) experimental SAS data
Myotilin Ig1Ig2 (220-452) Kratky plot
Sample: Myotilin Ig1Ig2 (220-452) monomer, 27 kDa Homo sapiens protein
Buffer: 20 mM Na+-HEPES, 150 mM, NaCl, 5 % v/v glycerol, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2010 Nov 16
Structural insights into F-actin regulation and sarcomere assembly via myotilin (2020)
Kostan J, Pavšič M, Puž V, Schwarz T, Drepper F, Molt S, Graewert M, Schreiner C, Sajko S, van der Ven P, Svergun D, Warscheid B, Konrat R, Fürst D, Lenarčič B, Djinović-Carugo K
RgGuinier 3.7 nm
Dmax 15.1 nm
VolumePorod 52 nm3

SASDF38 – Myotilin immunoglobulin domains Ig1Ig2 (250-444)

UniProt ID: Q9UBF9 (250-444) Myotilin Ig1Ig2 (250-444)

Myotilin Ig1Ig2 (250-444) experimental SAS data
Myotilin Ig1Ig2 (250-444) Kratky plot
Sample: Myotilin Ig1Ig2 (250-444) monomer, 22 kDa Homo sapiens protein
Buffer: 20 mM Na+-HEPES, 150 mM, NaCl, 5 % v/v glycerol, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2019 Jun 27
Structural insights into F-actin regulation and sarcomere assembly via myotilin (2020)
Kostan J, Pavšič M, Puž V, Schwarz T, Drepper F, Molt S, Graewert M, Schreiner C, Sajko S, van der Ven P, Svergun D, Warscheid B, Konrat R, Fürst D, Lenarčič B, Djinović-Carugo K
RgGuinier 2.8 nm
Dmax 10.1 nm
VolumePorod 29 nm3

SASDF48 – Myotilin immunoglobulin domains Ig1Ig2 (250-498)

UniProt ID: Q9UBF9 (250-498) Myotilin Ig1Ig2 (250-498)

Myotilin Ig1Ig2 (250-498) experimental SAS data
Myotilin Ig1Ig2 (250-498) Kratky plot
Sample: Myotilin Ig1Ig2 (250-498) monomer, 28 kDa Homo sapiens protein
Buffer: 20 mM Na+-HEPES, 150 mM, NaCl, 5 % v/v glycerol, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2019 Jun 27
Structural insights into F-actin regulation and sarcomere assembly via myotilin (2020)
Kostan J, Pavšič M, Puž V, Schwarz T, Drepper F, Molt S, Graewert M, Schreiner C, Sajko S, van der Ven P, Svergun D, Warscheid B, Konrat R, Fürst D, Lenarčič B, Djinović-Carugo K
RgGuinier 3.2 nm
Dmax 14.0 nm
VolumePorod 37 nm3

SASDJH8 – Myotilin immunoglobulin domains Ig1Ig2 (220-452, wild-type) concentration series data

UniProt ID: Q9UBF9 (220-452) Myotilin (222-452)

Myotilin (222-452) experimental SAS data
Myotilin (222-452) Kratky plot
Sample: Myotilin (222-452) monomer, 26 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5 % glycerol, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jul 6
Structural insights into F-actin regulation and sarcomere assembly via myotilin (2020)
Kostan J, Pavšič M, Puž V, Schwarz T, Drepper F, Molt S, Graewert M, Schreiner C, Sajko S, van der Ven P, Svergun D, Warscheid B, Konrat R, Fürst D, Lenarčič B, Djinović-Carugo K
RgGuinier 3.3 nm
Dmax 11.5 nm
VolumePorod 36 nm3

SASDJJ8 – Myotilin immunoglobulin domains Ig1Ig2 (220-452, R405K mutant) concentration series data

UniProt ID: Q9UBF9 (222-452) Myotilin (222-452) R405K

Myotilin (222-452) R405K experimental SAS data
Myotilin (222-452) R405K Kratky plot
Sample: Myotilin (222-452) R405K monomer, 26 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5 % glycerol, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jul 12
Structural insights into F-actin regulation and sarcomere assembly via myotilin (2020)
Kostan J, Pavšič M, Puž V, Schwarz T, Drepper F, Molt S, Graewert M, Schreiner C, Sajko S, van der Ven P, Svergun D, Warscheid B, Konrat R, Fürst D, Lenarčič B, Djinović-Carugo K
RgGuinier 3.3 nm
Dmax 10.5 nm
VolumePorod 36 nm3