SASBDB entries for UniProt ID: Q9X721

SASDCL2 – Tandem CBD from Clostridium histolyticum ColG collagenase at pCa 3

UniProt ID: Q9X721 (883-1118) Class1 collagenase
Class1 collagenase experimental SAS data
DAMMIF model
Sample: Class1 collagenase monomer, 27 kDa Hathewaya histolytica protein
Buffer: 10 mM HEPES 100 mM NaCL, 2% glycerol, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Sep 14
Structural characterization of bacterial collagenases University of Arkansas PhD thesis 2431 (2017)
Bauer R
RgGuinier 2.4 nm
Dmax 7.5 nm
VolumePorod 36 nm3

SASDCM2 – Tandem CBD from Clostridium histolyticum ColG collagenase at pCa 4

UniProt ID: Q9X721 (883-1118) Class1 collagenase collagen-binding domain
Class1 collagenase collagen-binding domain experimental SAS data
DAMMIF model
Sample: Class1 collagenase collagen-binding domain monomer, 27 kDa Hathewaya histolytica protein
Buffer: 10 mM HEPES 100 mM NaCL, 2% glycerol, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Sep 14
Structural characterization of bacterial collagenases University of Arkansas PhD thesis 2431 (2017)
Bauer R
RgGuinier 2.4 nm
Dmax 9.8 nm
VolumePorod 33 nm3

SASDCN2 – Tandem CBD from Clostridium histolyticum ColG collagenase at pCa 5

UniProt ID: Q9X721 (883-1118) Class1 collagenase
Class1 collagenase experimental SAS data
DAMMIF model
Sample: Class1 collagenase monomer, 27 kDa Hathewaya histolytica protein
Buffer: 10 mM HEPES 100 mM NaCL, 2% glycerol, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Sep 14
Structural characterization of bacterial collagenases University of Arkansas PhD thesis 2431 (2017)
Bauer R
RgGuinier 2.5 nm
Dmax 9.4 nm
VolumePorod 36 nm3

SASDCP2 – Tandem CBD from Clostridium histolyticum ColG collagenase at pCa 6

UniProt ID: Q9X721 (883-1118) Class1 collagenase
Class1 collagenase experimental SAS data
DAMMIF model
Sample: Class1 collagenase monomer, 27 kDa Hathewaya histolytica protein
Buffer: 10 mM HEPES 100 mM NaCL, 2% glycerol, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Sep 14
Structural characterization of bacterial collagenases University of Arkansas PhD thesis 2431 (2017)
Bauer R
RgGuinier 2.6 nm
Dmax 12.8 nm
VolumePorod 33 nm3

SASDC64 – Envelope of Col G PKD-CBD-CBD complexed with mini-collagen

UniProt ID: (None-None) Collagenous Peptide model [(PPG)10]

UniProt ID: Q9X721 (786-1118) ColG Collagenase
Collagenous Peptide model [(PPG)10]ColG Collagenase experimental SAS data
DAMMIF model
Sample: Collagenous Peptide model [(PPG)10] trimer, 9 kDa synthetic construct protein
ColG Collagenase monomer, 37 kDa Hathewaya histolytica protein
Buffer: 50 mM HEPES, 100 mM NaCl, 5 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Oct 12
Ca2+ -induced orientation of tandem collagen binding domains from clostridial collagenase ColG permits two opposing functions of collagen fibril formation and retardation. FEBS J 285(17):3254-3269 (2018)
Caviness P, Bauer R, Tanaka K, Janowska K, Roeser JR, Harter D, Sanders J, Ruth C, Matsushita O, Sakon J
RgGuinier 4.1 nm
Dmax 19.3 nm
VolumePorod 70 nm3

SASDCS4 – Collagenase ColG s2s3as3b at pCa 3

UniProt ID: Q9X721 (787-1118) Collagenase ColG segement s2s3as3b
Collagenase ColG segement s2s3as3b experimental SAS data
DAMMIF model
Sample: Collagenase ColG segement s2s3as3b monomer, 37 kDa Hathewaya histolytica protein
Buffer: 10mM HEPES 100mM NaCl 0.2mM EGTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Oct 12
Ca2+ - Induced Structural Change of Multi-Domain Collagen Binding Segments of Collagenases ColG and ColH from Hathewaya histolytica University of Arkansas Dissertation - (2018)
Christopher E Ruth
RgGuinier 3.0 nm
Dmax 12.7 nm
VolumePorod 52 nm3

SASDD63 – Collagenase ColG s2s3as3b at pCa 3 (second analysis)

UniProt ID: Q9X721 (787-1118) Collagenase ColG segement s2s3as3b
Collagenase ColG segement s2s3as3b experimental SAS data
DAMFILT model
Sample: Collagenase ColG segement s2s3as3b monomer, 37 kDa Hathewaya histolytica protein
Buffer: 10mM HEPES 100mM NaCl 0.2mM EGTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Oct 11
Ca2+ - Induced Structural Change of Multi-Domain Collagen Binding Segments of Collagenases ColG and ColH from Hathewaya histolytica University of Arkansas Dissertation - (2018)
Christopher E Ruth
RgGuinier 3.0 nm
Dmax 14.4 nm
VolumePorod 61 nm3

SASDCT4 – Collagenase ColG s2s3as3b at pCa 4

UniProt ID: Q9X721 (787-1118) Collagenase ColG segement s2s3as3b
Collagenase ColG segement s2s3as3b experimental SAS data
DAMMIF model
Sample: Collagenase ColG segement s2s3as3b monomer, 37 kDa Hathewaya histolytica protein
Buffer: 10mM HEPES 100mM NaCl 0.2mM EGTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Oct 12
Ca2+ - Induced Structural Change of Multi-Domain Collagen Binding Segments of Collagenases ColG and ColH from Hathewaya histolytica University of Arkansas Dissertation - (2018)
Christopher E Ruth
RgGuinier 3.3 nm
Dmax 11.5 nm
VolumePorod 63 nm3

SASDCU4 – Collagenase ColG s2s3as3b at pCa 5

UniProt ID: Q9X721 (787-1118) Collagenase ColG segement s2s3as3b
Collagenase ColG segement s2s3as3b experimental SAS data
DAMMIF model
Sample: Collagenase ColG segement s2s3as3b monomer, 37 kDa Hathewaya histolytica protein
Buffer: 10mM HEPES 100mM NaCl 0.2mM EGTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Oct 12
Ca2+ - Induced Structural Change of Multi-Domain Collagen Binding Segments of Collagenases ColG and ColH from Hathewaya histolytica University of Arkansas Dissertation - (2018)
Christopher E Ruth
RgGuinier 4.3 nm
Dmax 19.7 nm
VolumePorod 98 nm3

SASDCV4 – Collagenase ColG s2s3as3b at pCa 6

UniProt ID: Q9X721 (787-1118) Collagenase ColG segement s2s3as3b
Collagenase ColG segement s2s3as3b experimental SAS data
DAMMIF model
Sample: Collagenase ColG segement s2s3as3b monomer, 37 kDa Hathewaya histolytica protein
Buffer: 10mM HEPES 100mM NaCl 0.2mM EGTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Oct 12
Ca2+ - Induced Structural Change of Multi-Domain Collagen Binding Segments of Collagenases ColG and ColH from Hathewaya histolytica University of Arkansas Dissertation - (2018)
Christopher E Ruth
RgGuinier 3.6 nm
Dmax 15.2 nm
VolumePorod 41 nm3