SASBDB entries for UniProt ID: Q9Y5P4

SASDNC7 – miniCERTL with N-terminal His-tag

UniProt ID: Q9Y5P4 (None-None) Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624)
Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) experimental SAS data
GASBOR model
Sample: Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) monomer, 45 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Sep 25
Herpes simplex virus 1 protein pUL21 alters ceramide metabolism by activating the inter-organelle transport protein CERT Journal of Biological Chemistry :102589 (2022)
Benedyk T, Connor V, Caroe E, Shamin M, Svergun D, Deane J, Jeffries C, Crump C, Graham S
RgGuinier 2.7 nm
Dmax 9.1 nm
VolumePorod 71 nm3

SASDND7 – Complex of H6-pUL21C and H6-miniCERTL

UniProt ID: P10205 (275-535) Tegument protein UL21 (C-terminal domain)

UniProt ID: Q9Y5P4 (None-None) Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624)
Tegument protein UL21 (C-terminal domain)Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) experimental SAS data
GASBOR model
Sample: Tegument protein UL21 (C-terminal domain) monomer, 29 kDa Human herpesvirus 1 … protein
Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) monomer, 45 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Sep 25
Herpes simplex virus 1 protein pUL21 alters ceramide metabolism by activating the inter-organelle transport protein CERT Journal of Biological Chemistry :102589 (2022)
Benedyk T, Connor V, Caroe E, Shamin M, Svergun D, Deane J, Jeffries C, Crump C, Graham S
RgGuinier 3.3 nm
Dmax 13.6 nm
VolumePorod 84 nm3

SASDNE7 – Complex of H6-miniCERTL and full-length pUL21-H6

UniProt ID: P10205 (1-535) Tegument protein UL21

UniProt ID: Q9Y5P4 (None-None) Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624)
Tegument protein UL21Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) experimental SAS data
GASBOR model
Sample: Tegument protein UL21 monomer, 59 kDa Human alphaherpesvirus 1 … protein
Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) monomer, 45 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Sep 25
Herpes simplex virus 1 protein pUL21 alters ceramide metabolism by activating the inter-organelle transport protein CERT Journal of Biological Chemistry :102589 (2022)
Benedyk T, Connor V, Caroe E, Shamin M, Svergun D, Deane J, Jeffries C, Crump C, Graham S
RgGuinier 4.1 nm
Dmax 15.8 nm
VolumePorod 158 nm3