Browse by MODEL: Ensemble

SASDAR3 – PsrP functional binding region

Functional binding region (187-385) of the pneumococcal serine-rich repeat protein experimental SAS data
PsrP functional binding region Rg histogram
Sample: Functional binding region (187-385) of the pneumococcal serine-rich repeat protein monomer, 22 kDa Streptococcus pneumoniae protein
Buffer: 20 mM sodium citrate 250 mM NaCl 2.5 % Glycerol, pH: 5.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Jul 2
The basic keratin 10-binding domain of the virulence-associated pneumococcal serine-rich protein PsrP adopts a novel MSCRAMM fold. Open Biol 4:130090 (2014)
Schulte T, Löfling J, Mikaelsson C, Kikhney A, Hentrich K, Diamante A, Ebel C, Normark S, Svergun D, Henriques-Normark B, Achour A
RgGuinier 2.3 nm
Dmax 7.7 nm
VolumePorod 37 nm3

SASDLT5 – Modification methylase SsoII (M.SsoII) protein bound to 12-bp DNA

Modification methylase SsoII12-bp DNA experimental SAS data
Modification methylase SsoII (M.SsoII) protein bound to 12-bp DNA Rg histogram
Sample: Modification methylase SsoII monomer, 43 kDa Shigella sonnei protein
12-bp DNA monomer, 8 kDa DNA
Buffer: 50 mM Na-phosphate buffer, pH: 7
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Mar 13
Flexibility of the linker between the domains of DNA methyltransferase SsoII revealed by small-angle X-ray scattering: implications for transcription regulation in SsoII restriction-modification system. PLoS One 9(4):e93453 (2014)
Konarev PV, Kachalova GS, Ryazanova AY, Kubareva EA, Karyagina AS, Bartunik HD, Svergun DI
RgGuinier 2.8 nm
Dmax 11.0 nm
VolumePorod 85 nm3

SASDBR4 – Leishmania braziliensis Activator of Hsp90 ATPase-1 (LbAha1)

Activator of Hsp90 ATPase-1 experimental SAS data
Leishmania braziliensis Activator of Hsp90 ATPase-1 (LbAha1) Rg histogram
Sample: Activator of Hsp90 ATPase-1 monomer, 38 kDa Leishmania braziliensis protein
Buffer: 25 mM Sodium phosphate, 50 mM NaCl, 2 mM EDTA, 1 mM β-mercaptoethanol, pH: 7
Experiment: SAXS data collected at SAXS2 Beamline, Brazilian Synchrotron Light Laboratory on 2013 Jun 1
Low resolution structural studies indicate that the activator of Hsp90 ATPase 1 (Aha1) of Leishmania braziliensis has an elongated shape which allows its interaction with both N- and M-domains of Hsp90. PLoS One 8(6):e66822 (2013)
Seraphim TV, Alves MM, Silva IM, Gomes FE, Silva KP, Murta SM, Barbosa LR, Borges JC
RgGuinier 3.6 nm
Dmax 14.5 nm

SASDLL5 – frataxin homolog, Yfh1, at 0 °C

Frataxin homolog, mitochondrial experimental SAS data
frataxin homolog, Yfh1, at 0 °C Rg histogram
Sample: Frataxin homolog, mitochondrial monomer, 14 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 20 mM HEPES, pH: 7
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2008 Nov 28
The role of hydration in protein stability: comparison of the cold and heat unfolded states of Yfh1. J Mol Biol 417(5):413-24 (2012)
Adrover M, Martorell G, Martin SR, Urosev D, Konarev PV, Svergun DI, Daura X, Temussi P, Pastore A
RgGuinier 2.2 nm

SASDLM5 – frataxin homolog, Yfh1, at 20 °C

Frataxin homolog, mitochondrial experimental SAS data
frataxin homolog, Yfh1, at 20 °C Rg histogram
Sample: Frataxin homolog, mitochondrial monomer, 14 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 20 mM HEPES, pH: 7
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2008 Nov 28
The role of hydration in protein stability: comparison of the cold and heat unfolded states of Yfh1. J Mol Biol 417(5):413-24 (2012)
Adrover M, Martorell G, Martin SR, Urosev D, Konarev PV, Svergun DI, Daura X, Temussi P, Pastore A
RgGuinier 2.1 nm

SASDLN5 – frataxin homolog, Yfh1, at 50 °C

Frataxin homolog, mitochondrial experimental SAS data
frataxin homolog, Yfh1, at 50 °C Rg histogram
Sample: Frataxin homolog, mitochondrial monomer, 14 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 20 mM HEPES, pH: 7
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2008 Nov 28
The role of hydration in protein stability: comparison of the cold and heat unfolded states of Yfh1. J Mol Biol 417(5):413-24 (2012)
Adrover M, Martorell G, Martin SR, Urosev D, Konarev PV, Svergun DI, Daura X, Temussi P, Pastore A
RgGuinier 2.5 nm

SASDED2 – Polyglutamine tract-binding protein 1 (PQBP-1)

Polyglutamine-binding protein 1 experimental SAS data
Polyglutamine tract-binding protein 1 (PQBP-1) Rg histogram
Sample: Polyglutamine-binding protein 1 monomer, 31 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 1mM DTT,, pH: 7
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Nov 18
Solution model of the intrinsically disordered polyglutamine tract-binding protein-1. Biophys J 102(7):1608-16 (2012)
Rees M, Gorba C, de Chiara C, Bui TT, Garcia-Maya M, Drake AF, Okazawa H, Pastore A, Svergun D, Chen YW
RgGuinier 3.7 nm
Dmax 13.0 nm
VolumePorod 51 nm3

SASDLX4 – Full-length flavorubredoxin

Anaerobic nitric oxide reductase flavorubredoxin experimental SAS data
Full-length flavorubredoxin Rg histogram
Sample: Anaerobic nitric oxide reductase flavorubredoxin tetramer, 217 kDa Escherichia coli (strain … protein
Buffer: 50 mM Tris-HCl, 18% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Nov 19
Quaternary Structure of Flavorubredoxin as Revealed by Synchrotron Radiation Small-Angle X-Ray Scattering Structure 16(9):1428-1436 (2008)
Petoukhov M, Vicente J, Crowley P, Carrondo M, Teixeira M, Svergun D
RgGuinier 4.1 nm
Dmax 14.2 nm
VolumePorod 297 nm3

SASDNC2 – NNGH-inhibited, cadmium(II)-substituted Phe171Asp/Glu219Ala double mutant of FL-MMP- 12

Macrophage metalloelastase experimental SAS data
NNGH-inhibited, cadmium(II)-substituted Phe171Asp/Glu219Ala double mutant of FL-MMP- 12 Rg histogram
Sample: Macrophage metalloelastase monomer, 54 kDa Homo sapiens protein
Buffer: 20 mM Tris10 mM CaCl2, 0.3 M NaCl, 0.2 M AHA, pH: 7.2
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Oct 2
Evidence of Reciprocal Reorientation of the Catalytic and Hemopexin-Like Domains of Full-Length MMP-12 Journal of the American Chemical Society 130(22):7011-7021 (2008)
Bertini I, Calderone V, Fragai M, Jaiswal R, Luchinat C, Melikian M, Mylonas E, Svergun D
RgGuinier 3.2 nm
Dmax 11.0 nm
VolumePorod 58 nm3